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Indirect immunofluorescence staining of rat retinal dopaminergic neurons using Zymed Rb anti-phospho-Tyrosine Hydroxylase (Ser31) (Cat. No. 36-9900). Image courtesy of Paul Witkovsky, PhD, Dept. of Ophthalmology, New York University School of Medicine.
|Tested species reactivity||Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho peptide derived from a region of the rat tyrosine hydroxylase (TH) protein surrounding the phosphorylated Ser31 residue.|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). It is responsible for the conversion of L-tyrosine to L-dopa in the catecholamine synthesis pathway
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Rat||Not Cited||Progesterone decreases tyrosine hydroxylase phosphorylation state and increases protein phosphatase 2A activity in the stalk-median eminence on proestrous afternoon.||Liu B,Arbogast LA||The Journal of endocrinology (204:209)||2010|
Phosphorylation state of tyrosine hydroxylase in the stalk-median eminence is decreased by progesterone in cycling female rats.
36-9900 was used in western blot to study the role of tyrosine hydroxylase in the stalk-median eminence of female rats.
|Liu B,Arbogast LA||Endocrinology (149:1462)||2008|
|Mouse||1:10000||Regulation of dopaminergic transmission and cocaine reward by the Clock gene.||McClung CA,Sidiropoulou K,Vitaterna M,Takahashi JS,White FJ,Cooper DC,Nestler EJ||Proceedings of the National Academy of Sciences of the United States of America (102:9377)||2005|
EC 18.104.22.168; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase