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Indirect immunofluorescence staining of rat retinal dopaminergic neurons using Zymed Rb anti-phospho-Tyrosine Hydroxylase (Ser40) (Cat. No. 36-8600). Image courtesy of Paul Witkovsky, PhD, Dept. of Ophthalmology, New York University School of Medicine.
|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide derived from the rat tyrosine hydroxylase (TH) protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/ml BSA, 0.15M NaCl|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). It is responsible for the conversion of L-tyrosine to L-dopa in the catecholamine synthesis pathway
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Litter size reduction alters insulin signaling in the ventral tegmental area and influences dopamine-related behaviors in adult rats.
36-8600 was used in western blot to investigate the effect of early postnatal overfeeding on insulin signaling in the ventral tegmental area.
|Portella AK,Silveira PP,Laureano DP,Cardoso S,Bittencourt V,Noschang C,Werlang I,Fontella FU,Dalmaz C,Goldani MZ||Behavioural brain research (278:66)||2015|
|Rat||Not Cited||Progesterone decreases tyrosine hydroxylase phosphorylation state and increases protein phosphatase 2A activity in the stalk-median eminence on proestrous afternoon.||Liu B,Arbogast LA||The Journal of endocrinology (204:209)||2010|
Phosphorylation state of tyrosine hydroxylase in the stalk-median eminence is decreased by progesterone in cycling female rats.
36-8600 was used in western blot to study the role of tyrosine hydroxylase in the stalk-median eminence of female rats.
|Liu B,Arbogast LA||Endocrinology (149:1462)||2008|
EC 18.104.22.168; TH isoform 3; TH isoform a; TH-4; TY3H; TYH; tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; tyrosine hydroxylase