This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
On western blotting this antibody detects a protein with the apparent MW of 200 kDa. Positive controls used Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR2, or 293T cells transfected with full length VEGFR2 and stimulated with 10 ng/mL VEGF for 5 minutes.
VEGFR3 has not been tested, but is expected to react with this antibody.
VEGFR2 (vascular endothelial growth factor receptor 2, also known as FLK-1/KDR) is a cell-membrane receptor tyrosine kinase with a high-affinity for its ligand, vascular endothelial growth factor (VEGF). VEGFR2 is putatively involved in the growth of endothelial cells and angiogenesis and plays an essential role in embryonic development, wound healing, cell proliferation, migration, and differentiation. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4. VEGFR2 dimerizes and is autophosphorylated on multiple tyrosine residues upon ligand binding. These phosphotyrosine sites are involved in regulation of intrinsic tyrosine kinase activity, or serve as binding sites for SH2 domains and phosphotyrosine binding (PTB) domains of downstream signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity.
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Protein Aliases: 6130401C07; CD309; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; Flk1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); kinase NYK; Krd-1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble vascular endothelial growth factor receptor 2; soluble VEGFR2; sVEGFR-2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF receptor-2; VEGFR-2; VEGFR2
Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2
UniProt ID: (Human) P35968