Western blot analysis was performed on whole cell extracts (30 ug lysate) of HT-29 (Lane 1), HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/ml of SCF (Lane 2), CaCo-2 (Lane 3) and CaCo-2 Serum Starved followed by treatment for 10 minutes with 100 ng/ml of SCF (Lane 4). The blots were probed with Anti-VEGF Receptor 2 [pY1214] Rabbit Polyclonal Antibody (Product# 441052, 0.35-1 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G21234,1:5000 dilution). A 151 kDa band corresponding to VEGF Receptor 2 [pY1214] was enhanced upon treatment across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosine 1214. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||0.35-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Flk-1/KDR vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The docking protein FRS2¿ is a critical regulator of VEGF receptors signaling.
44-1052 was used in western blot to show that FRS2α is a component of the VEGF receptors.
|Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M||Proceedings of the National Academy of Sciences of the United States of America (111:5514)||2014|
Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.
44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions
|Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC||PloS one (5:null)||2010|
|Not Applicable||Not Cited||
Microtubules coordinate VEGFR2 signaling and sorting.
44-1052 was used in western blot to study intracellular sorting of VEGFR2 after ligand stimulation
|Czeisler C,Mikawa T||PloS one (8:null)||2013|
|Human||Not Cited||The important roles of RET, VEGFR2 and the RAF/MEK/ERK pathway in cancer treatment with sorafenib.||Mao WF,Shao MH,Gao PT,Ma J,Li HJ,Li GL,Han BH,Yuan CG||Acta pharmacologica Sinica (33:1311)||2012|