Invitrogen

Phospho-VEGF Receptor 2 (Tyr1214) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

9 References

View all (64) FLK1 (VEGF Receptor 2) antibodies

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Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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Phospho-VEGF Receptor 2 (Tyr1214) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF (Lane 2), CaCo-2 (Lane 3) and CaCo-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF (Lane 4). The blots were probed with Anti-VEGF Receptor 2 (pY1214) Rabbit Polyclonal Antibody (Product # 44-1052, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 151 kDa band corresponding to VEGF Receptor 2 (pY1214) was enhanced upon treatment across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Phospho-VEGF Receptor 2 (Tyr1214) Antibody in Immunocytochemistry (ICC/IF) — Figure 6 FGF2- and VEGF 165 -induced peripheral accumulation of activated FAK and F-actin is inhibited by 2SNS 12-mer oligosaccharide. ( A ) Lack of peripheral accumulation of FAK phosphorylated on tyrosine 397 and F-actin in serum-starved HUVECs. Cells were co-stained with anti-phospho-FAK (pTyr397) antibody (left image) and phalloidin-AlexaFluor568 (middle image). Merged view is represented in the right image. Scale bar, 75 um. ( B-C ) Peripheral FAK phosphorylated at tyrosine 397 and F-actin are detected after 10 min stimulation with FGF2 ( B , upper images) or VEGF 165 ( C , upper images). 12-mer 2SNS oligosaccharide prevents peripheral localization of phosphorylated FAK and F-actin in response to FGF2 ( B , lower images) or VEGF 165 ( C , lower images). Merged images are presented in the right panel. Scale bars, 75 um. ( D ) Percentage of cells with peripheral FAK phosphorylated on tyrosine 397 in unstimulated (0.1% FBS) or FGF2 or VEGF 165 stimulated cells in the absence or presence of 12-mer 2SNS or 12-mer 2S is shown as the mean +-SD (n = 3). *, P <0.0005; +, P <0.01.

Phospho-VEGF Receptor 2 (Tyr1214) Antibody — Modulation of expression of target protein by cell treatment to demonstrate antibody specificity. Western blotting analysis of Phospho-VEGF Receptor 2 (Tyr1214) using anti Phospho-VEGF Receptor 2 (Tyr1214) Polyclonal Antibody (Product # 44-1052) shows increased expression in HT29 and Caco2 cells treated with SCF.

Product Details

44-1052

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosine 1214. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2533558

Target Information

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells.

International journal of ophthalmology

Qian YY,Wu HY,Liu GQ,Ren C,Lu PR,Zhang XG

44-1052 was used in Western Blotting to investigate the effects of blockade of insulin receptor substrate-1 (IRS-1) on the bio-function of tube formation of human choroidal endothelial cells (HCECs).

Wed Sep 30 00:00:00 EDT 2020

Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling.

Cell death & disease

Baek M,Kim M,Lim JS,Morales LD,Hernandez J,Mummidi S,Williams-Blangero S,Jang IS,Tsin AT,Kim DJ

44-1052 was used in Western Blotting to suggest that T-cell protein tyrosine phosphatase is protective against UVB-induced keratinocyte cell damage by promoting apoptosis via negative regulation of Flk-1/JNK survival signalling.

Thu Jun 28 00:00:00 EDT 2018

Microtubules coordinate VEGFR2 signaling and sorting.

PloS one

Czeisler C,Mikawa T

44-1052 was used in western blot to study intracellular sorting of VEGFR2 after ligand stimulation

Tue May 27 00:00:00 EDT 2014

The docking protein FRS2α is a critical regulator of VEGF receptors signaling.

Proceedings of the National Academy of Sciences of the United States of America

Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M

44-1052 was used in western blot to show that FRS2α is a component of the VEGF receptors.

Tue Apr 15 00:00:00 EDT 2014

The docking protein FRS2α is a critical regulator of VEGF receptors signaling.

Proceedings of the National Academy of Sciences of the United States of America

Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M

44-1052 was used in western blot to show that FRS2α is a component of the VEGF receptors.

Tue Apr 15 00:00:00 EDT 2014

The important roles of RET, VEGFR2 and the RAF/MEK/ERK pathway in cancer treatment with sorafenib.

Acta pharmacologica Sinica

Mao WF,Shao MH,Gao PT,Ma J,Li HJ,Li GL,Han BH,Yuan CG

Mon Oct 01 00:00:00 EDT 2012

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 EDT 2010

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 EDT 2010

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2

UniProt ID: (Human) P35968, (Mouse) P35918

Entrez Gene ID: (Human) 3791, (Mouse) 16542

Molecular Function: transmembrane signal receptor