Peptide Competition and Stimulation: Extracts prepared from PAE cells transfected with a chimeric receptor and stimulated with CSF (1-4) or from NIH3T3 cells transfected with human VEGFR2 left unstimulated (5) or stimulated with VEGF (6) were resolved by
|Tested species reactivity||Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide from human VEGFR2 containing tyrosine 1054. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.35-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Flk-1/KDR vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
VEGFR2-PLCgamma1 axis is essential for endothelial specification of VEGFR2+ vascular progenitor cells.
44-1046 was used in western blot to investigate the signal transduction pathway downstream of VEGFR2 responsible for endothelial differentiation
|Sase H,Watabe T,Kawasaki K,Miyazono K,Miyazawa K||Journal of cell science (122:3303)||2009|
|Human||Not Cited||Novel role of ARF6 in vascular endothelial growth factor-induced signaling and angiogenesis.||Ikeda S,Ushio-Fukai M,Zuo L,Tojo T,Dikalov S,Patrushev NA,Alexander RW||Circulation research (96:467)||2005|
||Novel role of ARF6 in vascular endothelial growth factor-induced signaling and angiogenesis.||Ikeda S,Ushio-Fukai M,Zuo L,Tojo T,Dikalov S,Patrushev NA,Alexander RW||Circulation research (96:467)||2005|