Immunofluorescence analysis of Phospho-VEGF Receptor 2 pTyr1054 / pTyr1059 was done on 90% confluent log phase HUVEC cell treated with 100ng of VEGF for 30 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-VEGF Receptor 2 pTyr1054 / pTyr1059 Rabbit Polyclonal Antibody (441047G) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing punctuated membranous localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosines 1054 and 1059. The sequence is conserved among multiple species including mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Flk-1/KDR vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex.
44-1047G was used in western blot to study the role of VE-cadherin in fluid shear stress
|Coon BG,Baeyens N,Han J,Budatha M,Ross TD,Fang JS,Yun S,Thomas JL,Schwartz MA||The Journal of cell biology (208:975)||2015|
|Human||Not Cited||Investigation of the potential utility of a linomide analogue for treatment of choroidal neovascularization.||Abdel-Rahman MH,Yang Y,Salem MM,Meadows S,Massengill JB,Li PK,Davidorf FH||Experimental eye research (91:837)||2010|
The docking protein FRS2¿ is a critical regulator of VEGF receptors signaling.
44-1047G was used in western blot to show that FRS2α is a component of the VEGF receptors.
|Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M||Proceedings of the National Academy of Sciences of the United States of America (111:5514)||2014|