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Invitrogen
Phospho-XRCC4 (Ser260) Polyclonal Antibody
FIGURE: 1 / 1
Phospho-XRCC4 (Ser260) Antibody (PA5-64731) in WB
Nucleic acids research 2023 -
Figure 5. Deacetylation by SIRT2 directs DNA-PK activation and signaling. ( A ) WT and SIRT2 KO U2OS cells were treated with or without 10 Gy IR. Cells were fixed at 30, 60 or 90 min post IR and stained with anti-gammaH2AX (red), anti-DNA-PKcs pS2056 (green) and DAPI (blue). A representative 90 min post IR time point is shown. ( B ) Quantitation of the percentage of cells with >5 DNA-PKcs pS2056 foci is sho... View More
Product Details
PA5-64731
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human XRCC4 (Phospho-Ser260)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2663246
Product Specific Information
Phospho-XRCC4 (Ser260) Polyclonal Antibody detects endogenous levels of XRCC4 only when phosphorylated at Ser260.
Target Information
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA repair protein XRCC4; hXRCC4; X-ray repair complementing defective repair in Chinese hamster cells 4; X-ray repair cross-complementing protein 4
Gene Aliases: SSMED; XRCC4
UniProt ID: (Human) Q13426
Entrez Gene ID: (Human) 7518
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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