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Invitrogen
Phospho-ZAP70/Syk (Tyr493, Tyr526) Recombinant Rabbit Monoclonal Antibody (Zap70Y493-H11), FITC
FIGURE: 1 / 1
Phospho-ZAP70/Syk (Tyr493, Tyr526) Antibody (MA5-28071) in Flow
Flow cytometry of Phospho-Zap70 (Tyr493, Tyr526) using Jurkat cells. Samples were incubated in monoclonal Phospho-Zap70 (Tyr493, Tyr526) antibody (Product # MA5-36917). Images correspond with the following conditions: unstained, negative control (blue), stained and untreated (red), treated with pervanadate (green).
Product Details
MA5-28071
Product Specifications
Host/Isotype
Rabbit
/ IgG, kappa
Class
Recombinant Monoclonal
Type
Antibody
Clone
Zap70Y493-H11
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Storage conditions
4° C, store in dark
Shipping conditions
Wet ice
RRID
AB_2745062
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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