Immunofluorescent analysis of Phospho-Zap70 pTyr315/319 in Jurkat cells treated with H2O2 (top right, bottom) or untreated (top left) using a Phospho-Zap70 pTyr315/319 recombinant rabbit monoclonal antibody (Product # 700177) at a dilution of 2ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000 and nuclei stained using Hoescht (blue). Pre-incubation of treated cells with phosphopeptide immunogen decreased signal (bottom right) while pre-incubation with non-phosphopeptide did not (bottom right).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 311-323 of P43403.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-3 ug/test|
|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Western Blot (WB)||0.5-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with primate, bovine and Xenopus based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Zeta-associated protein (ZAP-70), a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T-cell antigen receptor. Phosphorylation of both tyrosines 315. Phosphorylation of tyrosines 315 and 319 plays an important role in mediating the positive and negative selection of T cells in thymus. TLR9-activated B-cells display elevated ZAP-70 expression levels which correlate with sustained PKB induction. Additionally,, a major catechin found in green tea, binds directly to ZAP-70 resulting in caspase-mediated apoptosis in Jurkat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.