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Western blot of rat cortex lysate showing specific labeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide (not shown).
|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 of rat SNCA conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, canine, human, mouse and non-human primate based on 100% sequence homology.
This antibody is specific for the ~15 kDa alpha-synuclein protein phosophorylated at Ser129. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson"e;s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Wild type and P301L mutant Tau promote neuro-inflammation and α-Synuclein accumulation in lentiviral gene delivery models.
PA1-4686 was used in western blot to investigate the effects of wild type and P301L mutant tau on neuro-inflammation and alpha-synuclein accumulation in a rat model
|Khandelwal PJ,Dumanis SB,Herman AM,Rebeck GW,Moussa CE||Molecular and cellular neurosciences (49:44)||2012|