Western blot analysis of Phospho-beta-1 Arrestin pSer412 in PC12 cell extracts, untreated (1, 3) or treated (2, 4) with PP2A phosphatase. Samples were probed with a Phospho-beta-1 Arrestin pSer412 polyclonal antibody (Product # PA1-26637) (lanes 3,4) and a pan non-phospho antibody (lanes 1,2).
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of rat beta Arrestin 1 containing serine 412.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26637 detects beta 1 Arrestin (phospho S412) from mouse and rat samples. PA1-26637 is expected to cross react with bovine (77% conserved) and human (77% conserved) due to sequence homology.
PA1-26637 has been successfully used in Western blot procedures.
The PA1-26637 immunogen is a synthetic phosphopeptide corresponding to a portion of rat beta Arrestin 1 containing serine 412.
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.