Western blot analysis of Phospho-beta-Catenin pTyr654 using Phospho-beta-Catenin pTyr654 polyclonal antibody (Product # PA5-36748) at a dilution of 1:500. Lane 1: DLD cell lysate treated with PMA, Lane 2: Mouse kidney tissue lysate, Lane 3: Rat kidney tissue lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Catenin-beta around the phosphorylation site of Tyrosine 654|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 92 kDa.
Purity is >95% by SDS-PAGE.
beta-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates beta-catenin at Ser45. This phosphorylation event primes beta-catenin for subsequent phosphorylation by GSK-3 beta. GSK-3 beta destabilizes beta-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of beta-catenin protein levels and have been found in many tumor cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.