Western blot analysis of Phospho-c-Fos pSer362 using Phospho-c-Fos pSer362 polyclonal antibody (Product # PA5-36769) at a dilution of 1:500. Lane 1: Hela cell lysate treated with PMA (100nM, 15min), Lane 2: NIH-3T3 cell lysate treated with PMA (100nM, 15min), Lane 3: PC12 cell lysate treated with PMA (100nM, 15min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Serine 362|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 62 kDa.
Purity is >95% by SDS-PAGE.
The c-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phospho-protein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.