Western blot detection of Phospho-c-Met pTyr1003 in extracts of H69 cells unstimulated (lane 1) or treated with 40 ng/mL HGF for 7.5 minutes (lanes 2-5). Samples were resolved by SDS-PAGE on a 10% polyacrylamide gel, transferred to PVDF, and blocked with a 5% BSA-TBST buffer, followed by incubation using a Phospho-c-Met pTyr1003 polyclonal antibody (Product # PA1-28461).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1003.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28461 detects c-Met from human samples.
PA1-28461 has been successfully used in Western blot applications.
The PA1-28461 immunogen is: Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1003.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.