Peptide Competition: Extracts prepared from 293T cells left unstimulated (1) or stimulated with HGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Dog, Virus, Hamster, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a trans-membrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Zebrafish cerebrospinal fluid mediates cell survival through a retinoid signaling pathway.
44-888G was used in immunohistochemistry to examine the contribution of cerebrospinal fluid during embryonic development.
|Chang JT,Lehtinen MK,Sive H||Developmental neurobiology (76:75)||2016|
Metastasis initiating cells in primary prostate cancer tissues from transurethral resection of the prostate (TURP) predicts castration-resistant progression and survival of prostate cancer patients.
44-888G was used in immunohistochemistry to examine the contribution of metastasis-initiating cells to prostate cancer.
|Li Q,Li Q,Nuccio J,Liu C,Duan P,Wang R,Jones LW,Chung LW,Zhau HE||The Prostate (75:1312)||2015|
Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms.
44-888G was used in western blot to suggest that HGF and EGFR signaling mediate ovarian cancer ascites-mediated migration of human peritoneal mesothelial cells
|Matte I,Lane D,Laplante C,Garde-Granger P,Rancourt C,Piché A||International journal of cancer (137:289)||2015|
Polycystin-1 and polycystin-2 are involved in the acquisition of aggressive phenotypes in colorectal cancer.
44-888G was used in immunohistochemistry to assess if PC1 and PC2 facilitate cancer aggressiveness.
|Gargalionis AN,Korkolopoulou P,Farmaki E,Piperi C,Dalagiorgou G,Adamopoulos C,Levidou G,Saetta A,Fragkou P,Tsioli P,Kiaris H,Zizi-Serbetzoglou A,Karavokyros I,Papavassiliou KA,Tsavaris N,Patsouris E,Basdra EK,Papavassiliou AG||International journal of cancer (136:1515)||2015|
Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-ß in gastric tissue.
44-888G was used in immunohistochemistry to use transgenic mice to determine the contribution of TGF-β1 to lymphocyte-mediated inflammation in gastrointestinal tumorigenesis.
|Ota M,Horiguchi M,Fang V,Shibahara K,Kadota K,Loomis C,Cammer M,Rifkin DB||Cancer research (74:2642)||2014|
In vitro and in vivo activity of cabozantinib (XL184), an inhibitor of RET, MET, and VEGFR2, in a model of medullary thyroid cancer.
44-888G was used in immunohistochemistry (paraffin) to study the biochemical activity of cabozantiinib against disease-associated RET mutants and its growth inhibitory activity in medullary thyroid cancer models.
|Bentzien F,Zuzow M,Heald N,Gibson A,Shi Y,Goon L,Yu P,Engst S,Zhang W,Huang D,Zhao L,Vysotskaia V,Chu F,Bautista R,Cancilla B,Lamb P,Joly AH,Yakes FM||Thyroid : official journal of the American Thyroid Association (23:1569)||2013|
MET expression in melanoma correlates with a lymphangiogenic phenotype.
44-888G was used in western blot to identify lymphangiogenic subpopulations from a melanoma of a single patient.
|Swoboda A,Schanab O,Tauber S,Bilban M,Berger W,Petzelbauer P,Mikula M||Human molecular genetics (21:3387)||2012|
MET and phosphorylated MET as potential biomarkers in lung cancer.
44-888G was used in immunohistochemistry to assess expression and prognostic role of the receptor tyrosine kinase MET, phosphorylated MET, and the ligand hepatocyte growth factor in patients with lung cancer.
|Tretiakova M,Salama AK,Karrison T,Ferguson MK,Husain AN,Vokes EE,Salgia R||Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer (30:341)||2011|
The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.
44-888G was used in immunocytochemistry and western blot to assess the contribution of c-Met signaling to dissemination of medulloblastoma
|Santhana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M||SpringerPlus (4:null)||2015|
Spatially restricted Hedgehog signalling regulates HGF-induced branching of the adult prostate.
44-888G was used in western blot to investigate the mechanisms underlying the specification of branch location
|Lim A,Shin K,Zhao C,Kawano S,Beachy PA||Nature cell biology (16:1135)||2014|
Inhibition of tumor growth and metastasis in non-small cell lung cancer by LY2801653, an inhibitor of several oncokinases, including MET.
44-888G was used in western blot to discuss the use of LY280653 in both preclinical in vitro and in vivo non-small cell lung cancer models.
|Wu W,Bi C,Credille KM,Manro JR,Peek VL,Donoho GP,Yan L,Wijsman JA,Yan SB,Walgren RA||Clinical cancer research : an official journal of the American Association for Cancer Research (19:5699)||2013|
Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer.
44-888G was used in western blot to compare multiplexed quantum dot labeling with other techniques to identify prostate cancer.
|Hu P,Chu GC,Zhu G,Yang H,Luthringer D,Prins G,Habib F,Wang Y,Wang R,Chung LW,Zhau HE||PloS one (6:null)||2011|
|Human||1:500||Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines.||Levin VA,Panchabhai SC,Shen L,Kornblau SM,Qiu Y,Baggerly KA||Journal of proteome research (9:179)||2010|
|Not Applicable||Not Cited||
The MET receptor tyrosine kinase is a potential novel therapeutic target for head and neck squamous cell carcinoma.
44-888G was used in western blot to study MET receptor tyrosine kinase as a potential novel therapeutic target for head and neck squamous cell carcinoma
|Seiwert TY,Jagadeeswaran R,Faoro L,Janamanchi V,Nallasura V,El Dinali M,Yala S,Kanteti R,Cohen EE,Lingen MW,Martin L,Krishnaswamy S,Klein-Szanto A,Christensen JG,Vokes EE,Salgia R||Cancer research (69:3021)||2009|
|Human||Not Cited||Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.||Bogatkevich GS,Ludwicka-Bradley A,Highland KB,Hant F,Nietert PJ,Singleton CB,Feghali-Bostwick CA,Silver RM||Arthritis and rheumatism (56:2432)||2007|
|Mouse||Not Cited||Prevention of neutrophil extravasation by hepatocyte growth factor leads to attenuations of tubular apoptosis and renal dysfunction in mouse ischemic kidneys.||Mizuno S,Nakamura T||The American journal of pathology (166:1895)||2005|
|Human||Not Cited||Functional expression and mutations of c-Met and its therapeutic inhibition with SU11274 and small interfering RNA in non-small cell lung cancer.||Ma PC,Jagadeeswaran R,Jagadeesh S,Tretiakova MS,Nallasura V,Fox EA,Hansen M,Schaefer E,Naoki K,Lader A,Richards W,Sugarbaker D,Husain AN,Christensen JG,Salgia R||Cancer research (65:1479)||2005|
|A selective small molecule inhibitor of c-Met kinase inhibits c-Met-dependent phenotypes in vitro and exhibits cytoreductive antitumor activity in vivo.||Christensen JG,Schreck R,Burrows J,Kuruganti P,Chan E,Le P,Chen J,Wang X,Ruslim L,Blake R,Lipson KE,Ramphal J,Do S,Cui JJ,Cherrington JM,Mendel DB||Cancer research (63:7345)||2003|
|Virus||Not Cited||Different susceptibility of human mesothelial cells to polyomavirus infection and malignant transformation.||Carbone M,Burck C,Rdzanek M,Rudzinski J,Cutrone R,Bocchetta M||Cancer research (63:6125)||2003|
|Mouse||Not Cited||A novel small molecule met inhibitor induces apoptosis in cells transformed by the oncogenic TPR-MET tyrosine kinase.||Sattler M,Pride YB,Ma P,Gramlich JL,Chu SC,Quinnan LA,Shirazian S,Liang C,Podar K,Christensen JG,Salgia R||Cancer research (63:5462)||2003|
Preservations of nephrin and synaptopodin by recombinant hepatocyte growth factor in podocytes for the attenuations of foot process injury and albuminuria in nephritic mice.
44-888G was used in immunohistochemistry - paraffin section to investigate the effect of hepatocyte growth factor on nephrin and synaptopodin expression.
|Kato T,Mizuno S,Nakamura T||Nephrology (Carlton, Vic.) (16:310)||2011|
|Not Applicable||Not Cited||
Expression patterns of PAX5, c-Met, and paxillin in neuroendocrine tumors of the lung.
44-888G was used in immunohistochemistry - paraffin section to examine the expression of PAX5, c-Met, and paxillin in in neuroendocrine tumors of the lung
|Song J,Li M,Tretiakova M,Salgia R,Cagle PT,Husain AN||Archives of pathology and laboratory medicine (134:1702)||2010|
Vascular endothelial growth factor regulates myeloid cell leukemia-1 expression through neuropilin-1-dependent activation of c-MET signaling in human prostate cancer cells.
44-888G was used in immunohistochemistry to study the regulation of Mcl-1 expression in prostate cancer cells
|Zhang S,Zhau HE,Osunkoya AO,Iqbal S,Yang X,Fan S,Chen Z,Wang R,Marshall FF,Chung LW,Wu D||Molecular cancer (9:null)||2010|
|Mouse||Not Cited||Hepatocyte growth factor contributes to fracture repair by upregulating the expression of BMP receptors.||Imai Y,Terai H,Nomura-Furuwatari C,Mizuno S,Matsumoto K,Nakamura T,Takaoka K||Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (20:1723)||2005|