Western blot detection of Phospho-c-Met pTyr1230 + pTyr1234 + pTyr1235 in extracts prepared from 293T cells left unstimulated (lane 1) or stimulated with HGF (lanes 2-5). Samples were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C and probed using a Phospho-c-Met pTyr1230 + pTyr1234 + pTyr1235 polyclonal antibody (Product # PA1-28463).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28463 detects c-Met from mouse, rat, and human samples.
PA1-28463 has been successfully used in Western blot applications.
The PA1-28463 immunogen is: Synthetic phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.