Western blot detection of Phospho-c-Met pTyr1349 in extracts prepared from 293T cells expressing human c-Met and stimulated with HGF. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C and probed using a Phospho-c-Met pTyr1349 polyclonal antibody (Product # PA1-28464).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1349.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28464 detects c-Met from human samples.
PA1-28464 has been successfully used in Western blot applications.
The PA1-28464 immunogen is: Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1349.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.