|Tested species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 338 and tyrosine 340. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26656 detects c-Raf (phospho S338 + Y340) from human samples. PA1-26656 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology.
PA1-26656 has been successfully used in Western blot procedures. The predicted molecular weight is 74 kDa. Suggested positive controls for this product are Immunoprecipitates or cell lysates from Hek293 cells transfected with c-Raf, or co-transfected with c-Raf, Ras[V12] and Rac 1.
The PA1-26656 immunogen is a synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 338 and tyrosine 340. The sequence is conserved in mouse and rat.
The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Phosphorylation of serine 338 creates a recognition site for tyrosine kinases and is critical for the activation of c-Raf. Serine 338 to tyrosine 341 (SSYY) is the phosphoregulatory site which regulates the biological function of the c-Raf tyrosine kinase by oncogenic Ras and Src.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Intracellular signaling cascade MAPKKK cascade Oncogenesis Oncogene Developmental processes Gametogenesis Oogenesis Spermatogenesis and motility Embryogenesis Anterior/posterior patterning Segment specification Cell cycle Cell cycle control Apoptosis Cell proliferation and differentiation