Whole cell lysates of Bend3 cells untreated (1) or treated with pervanadate (2) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse (monoclonal) anti-eNOS [pS1177] antibody (Catalog no. 44307M) at 1.0 µg/mL for 1 hour at 15-22°C. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detected using an ECL detection method (exposure time: 3 minutes).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Phosphopeptide derived from the region of endothelial nitric oxide synthase (eNOS) that contains serine 1177, conjugated to KLH.|
|Storage buffer||PBS, pH 7.3, with sucrose, PEG|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of tetraspanin CD151-¿3/¿6 integrin complex: Implication in angiogenesis CD151-integrin complex in angiogenesis.
44307M was used in western blot to determine the role and mechanism of CD151-integrin complex in angiogenesis.
|Liu WF,Zuo HJ,Chai BL,Peng D,Fei YJ,Lin JY,Yu XH,Wang DW,Liu ZX||The international journal of biochemistry and cell biology (43:642)||2011|
|Not Applicable||Not Cited||
CD151 gene delivery after myocardial infarction promotes functional neovascularization and activates FAK signaling.
44307M was used in western blot to elucidate how CD151 induces arteriogenesis and promotes functional neovascularization in a pig myocardial infarction model
|Zuo H,Liu Z,Liu X,Yang J,Liu T,Wen S,Zhang XA,Cianflone K,Wang D||Molecular medicine (Cambridge, Mass.) (15:307)||2009|