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|Tested species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer1177 of human eNOS|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
It is not recommended to aliquot this antibody.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of aromatase in sex-specific cerebrovascular endothelial function in mice.
PA5-17917 was used in western blot to study gender differences in murine cerebrovascular endothelial function and the role of aromatase
|Zuloaga KL,Davis CM,Zhang W,Alkayed NJ||American journal of physiology. Heart and circulatory physiology (306:H929)||2014|
Methylene blue modulates transendothelial migration of peripheral blood cells.
PA5-17917 was used in western blot to study the mechanisms underlying the modulation of peripheral blood cell transendothelial migration by methylene blue
|Werner I,Guo F,Bogert NV,Stock UA,Meybohm P,Moritz A,Beiras-Fernandez A||PloS one (8:null)||2013|
cNOS; constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183
2310065A03Rik; BOS_4740; cNOS; EC-NOS; ECNOS; eNOS; NOS; Nos-3; NOS3; NOSIII