Western blot analysis of Phospho-hnRNP K pSer284 using Phospho-hnRNP K pSer284 polyclonal antibody (Product # PA5-36811) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1ng/ml, 30min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1ng/ml, 30min), Lane 3: H9C2 cell lysate treated with EGF (0.1ng/ml, 30min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of Serine 284|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 51 kDa.
Purity is >95% by SDS-PAGE.
The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.