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|Tested species reactivity||Human|
|Published species reactivity||Rabbit , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Threonine187-phosphorylated p27 peptide derived from the C-terminus of the human p27 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Exposure of neuroblastoma cell lines to imatinib results in the upregulation of the CDK inhibitor p27(KIP1) as a consequence of c-Abl inhibition.
71-7700 was used in western blot to study the effect of imatinib on neuroblastoma cell lines
|Lupino E,Ramondetti C,Buccinnà B,Piccinini M||Biochemical pharmacology (92:235)||2014|
Human corneal endothelial cells employ phosphorylation of p27(Kip1) at both Ser10 and Thr187 sites for FGF-2-mediated cell proliferation via PI 3-kinase.
||Lee JG,Song JS,Smith RE,Kay EP||Investigative ophthalmology & visual science (52:8216)||2011|
Retinoic acid stabilizes p27Kip1 in EBV-immortalized lymphoblastoid B cell lines through enhanced proteasome-dependent degradation of the p45Skp2 and Cks1 proteins.
||Zancai P,Dal Col J,Piccinin S,Guidoboni M,Cariati R,Rizzo S,Boiocchi M,Maestro R,Dolcetti R||Oncogene (24:2483)||2005|
CDKN4, MEN4, KIP1, P27KIP1, MEN1B
cyclin-dependent kinase inhibitor 1B, CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, KIP1