Invitrogen

Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

2 Published Figures

11 References

View all (45) p38 MAPK alpha antibodies

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Datasheet

Questions & Answers

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Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Western Blot (WB) — Western blot analysis was performed by loading 30 ug ME-180 Cas9 (Lane 1), ME-180 Cas9 treated with TNF-alpha (50 ng/ml for 20 minutes) (lane 2), ME-180 TRADD KO (Lane 3), ME-180 TRADD KO treated with TNF-alpha (50 ng/ml for 20 minutes) (Lane 4) whole cell extracts. The blots were probed with Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8) (Product # 36-8500, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction in signal in CRISPR mediated knockout (KO) confirms that antibody is specific to Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8).

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Alzheimer's disease brain tissue using p38 MAPK (pT180/pY182) Polyclonal Antibody, Rabbit

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-p38 MAPK alpha (Thr180, Tyr182) was performed using 70% confluent log phase HeLa cells treated with UV for 40 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal Antibody (Product # 36-8500) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:100). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Reduction in signal was observed for target protein in TRADD knockout (KO) cell line using Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8) (Product # 36-8500). {KO}

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of Phospho-p38 MAPK alpha (Thr180, Tyr182) using Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal Antibody (Product # 36-8500) shows increased expression of proteins phosphorylated at the threonine and tyrosine residue in HeLa and A549 cell lines upon UV and TNF alpha treatments. {TM}

Phospho-p38 MAPK (Thr180, Tyr182) Antibody — Published figure using Phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (Product # 36-8500) in Western Blot

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250

Immunofluorescence (IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to a region surrounding the phosphorylated Thr180/Tyr182 residues of p38 MAP kinase.

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2533281

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primates and zebrafish based on 100% sequence homology.

Target Information

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; EC 2.7.11.24; ERK1; MAP 14; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK 14; MAPK1 antibody; MAX-interacting protein 2; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; RP1-179N16.5; SAPK2a; Stress-activated protein kinase 2a

Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38ALPHA; p38Hog; PRKM14; PRKM15; RK; SAPK2A

UniProt ID: (Human) Q16539

Entrez Gene ID: (Human) 1432, (Rat) 81649

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase