Invitrogen

Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

12 References

View all (64) p38 MAPK alpha antibodies

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Datasheet

Protocols

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Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-p38 MAPK alpha (Thr180, Tyr182) was performed using 70% confluent log phase HeLa cells treated with UV for 40 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal Antibody (Product # 36-8500) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:100). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Western Blot (WB) — Western blot analysis was performed by loading 30 ug ME-180 Cas9 (Lane 1), ME-180 Cas9 treated with TNF-alpha (50 ng/ml for 20 minutes) (lane 2), ME-180 TRADD KO (Lane 3), ME-180 TRADD KO treated with TNF-alpha (50 ng/ml for 20 minutes) (Lane 4) whole cell extracts. The blots were probed with Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8) (Product # 36-8500, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction in signal in CRISPR mediated knockout (KO) confirms that antibody is specific to Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8).

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Published figure using Phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (Product # 36-8500) in Western Blot and Immunohistochemistry

Phospho-p38 MAPK (Thr180, Tyr182) Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Reduction in signal was observed for target protein in TRADD knockout (KO) cell line using Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8) (Product # 36-8500).

Product Details

36-8500

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

1:250

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:250

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to a region surrounding the phosphorylated Thr180/Tyr182 residues of p38 MAP kinase.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2533281

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primates and zebrafish based on 100% sequence homology.

Target Information

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats.

Antioxidants (Basel, Switzerland)

Muraleva NA,Stefanova NA,Kolosova NG

36-8500 was used in Western Blotting to show that the mitochondrial antioxidant SkQ1 reduces the accumulation of Aβ in the hippocampus of senescence-accelerated OXYS rats during AD-like pathology by suppressing p38 MAPKsp activity.

Tue Jul 28 00:00:00 EDT 2020

SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats.

Antioxidants (Basel, Switzerland)

Muraleva NA,Stefanova NA,Kolosova NG

Tue Jul 28 00:00:00 EDT 2020

Methoxychlor and Vinclozolin Induce Rapid Changes in Intercellular and Intracellular Signaling in Liver Progenitor Cells.

Toxicological sciences : an official journal of the Society of Toxicology

Babica P,Zurabian R,Kumar ER,Chopra R,Mianecki MJ,Park JS,Jaša L,Trosko JE,Upham BL

368500 was used in western blot to determine the effects of methoxychlor and vinclozolin treatment on gap junctional intercellular communication and mitogen-activated protein kinase activation

Thu Sep 01 00:00:00 EDT 2016

Opposing effects of membrane-anchored CX3CL1 on amyloid and tau pathologies via the p38 MAPK pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lee S,Xu G,Jay TR,Bhatta S,Kim KW,Jung S,Landreth GE,Ransohoff RM,Lamb BT

36-8500 was used in western blot to investigate the role of membrane-anchored versus soluble CX3CL1 in regulating the microglia-mediated amelioration of Abeta pathology.

Wed Sep 10 00:00:00 EDT 2014

Opposing effects of membrane-anchored CX3CL1 on amyloid and tau pathologies via the p38 MAPK pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lee S,Xu G,Jay TR,Bhatta S,Kim KW,Jung S,Landreth GE,Ransohoff RM,Lamb BT

36-8500 was used in western blot to investigate the role of membrane-anchored versus soluble CX3CL1 in regulating the microglia-mediated amelioration of Abeta pathology.

Wed Sep 10 00:00:00 EDT 2014

Microglial derived tumor necrosis factor-α drives Alzheimer's disease-related neuronal cell cycle events.

Neurobiology of disease

Bhaskar K,Maphis N,Xu G,Varvel NH,Kokiko-Cochran ON,Weick JP,Staugaitis SM,Cardona A,Ransohoff RM,Herrup K,Lamb BT

36-8500 was used in western blot to investigate the role of microglial derived tumor necrosis factor alpha in neuronal cell cycle progression

Sat Feb 01 00:00:00 EST 2014

Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.

Proceedings of the National Academy of Sciences of the United States of America

Jang IK,Cronshaw DG,Xie LK,Fang G,Zhang J,Oh H,Fu YX,Gu H,Zou Y

Tue May 10 00:00:00 EDT 2011

T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.

Proceedings of the National Academy of Sciences of the United States of America

Adachi K,Davis MM

Tue Jan 25 00:00:00 EST 2011

T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.

Proceedings of the National Academy of Sciences of the United States of America

Adachi K,Davis MM

Tue Jan 25 00:00:00 EST 2011

Cell death or survival promoted by alternative isoforms of ErbB4.

Molecular biology of the cell

Sundvall M,Veikkolainen V,Kurppa K,Salah Z,Tvorogov D,van Zoelen EJ,Aqeilan R,Elenius K

Wed Dec 01 00:00:00 EST 2010

RhoA-GDP regulates RhoB protein stability. Potential involvement of RhoGDIalpha.

The Journal of biological chemistry

Ho TT,Merajver SD,Lapière CM,Nusgens BV,Deroanne CF

36-8500 was used in western blot to discuss mechanisms that regulate the stability of RhoGTPases

Fri Aug 01 00:00:00 EDT 2008

p38 Mitogen-activated protein kinase plays a stimulatory role in hepatic gluconeogenesis.

The Journal of biological chemistry

Cao W,Collins QF,Becker TC,Robidoux J,Lupo EG,Xiong Y,Daniel KW,Floering L,Collins S

Fri Dec 30 00:00:00 EST 2005

p38 Mitogen-activated protein kinase plays a stimulatory role in hepatic gluconeogenesis.

The Journal of biological chemistry

Cao W,Collins QF,Becker TC,Robidoux J,Lupo EG,Xiong Y,Daniel KW,Floering L,Collins S

Fri Dec 30 00:00:00 EST 2005

Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes.

Molecular and cellular biology

Robidoux J,Cao W,Quan H,Daniel KW,Moukdar F,Bai X,Floering LM,Collins S

Fri Jul 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; EC 2.7.11.24; ERK1; MAP 14; MAP kinase 14; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK1 antibody; MAX-interacting protein 2; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; RP1-179N16.5; SAPK2a; Stress-activated protein kinase 2a

Gene Aliases: CSBP; CSBP1; CSBP2; CSPB1; EXIP; MAPK14; MXI2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A

UniProt ID: (Human) Q16539

Entrez Gene ID: (Human) 1432

Molecular Function: non-receptor serine/threonine protein kinase

Function(s)

Process(es)

protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity protein binding ATP binding protein C-terminus binding enzyme binding protein phosphatase binding NFAT protein binding

Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Product Details

36-8500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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