Immunofluorescent analysis of Phospho-p38 MAPK pThr180/Tyr182 in HeLa cells -/+ UV light, using a Phospho-p38 MAPK pThr180/Tyr182 monoclonal antibody (Product # MA5-15177) showing absence of staining in untreated cells. Actin filaments are labeled with a fluorescent red phalloidin.
|Tested species reactivity||Fruit fly, Horse, Hamster, Human, Mink, Mouse, Non-human primate, Rat, Zebrafish|
|Published species reactivity||Zebrafish, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr180/Tyr182 of human p38 MAPK|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (NhpKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Duox1-derived H2O2 modulates Cxcl8 expression and neutrophil recruitment via JNK/c-JUN/AP-1 signaling and chromatin modifications.
MA5-15177 was used in immunohistochemistry - paraffin section to study the relationship between two neutrophil chemoattractants, DUOX1-derived hydrogen peroxide and CXCL8
|de Oliveira S,Boudinot P,Calado Â,Mulero V||Journal of immunology (Baltimore, Md. : 1950) (194:1523)||2015|
Myosin light chain kinase is responsible for high proliferative ability of breast cancer cells via anti-apoptosis involving p38 pathway.
MA5-15177 was used in western blot to investigate the effect of myosin light chain kinase on the proliferation of breast cancer cells and its mechanism
|Cui WJ,Liu Y,Zhou XL,Wang FZ,Zhang XD,Ye LH||Acta pharmacologica Sinica (31:725)||2010|