|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated Thr180 and Tyr182.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.01% BSA, 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with bovine, chicken, dog, monkey, mouse and zebrafish based on sequence homology.
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM NaF should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. In Western blot, this antibody detects a band at ~39kDa in Western blots of anisomycin C-6 glioma cell lysates.
p38 MAPK is a serine/threonin kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation. The protein is activated by phosphorylation of threonine 180 and tyrosine 182, by serveral upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation and endotoxin exposure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.