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Immunohistochemistry analysis of ERK1/2 [pTpY185/187] showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a ERK1/2 [pTpY185/187] polyclonal antibody (44680G) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, C. elegans, Chicken, Fruit fly, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Rat, C. elegans, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death.
44-680G was used in western blot to show that inhibition of the oxidative arm of the pentose phosphate pathway is required for antimalarial-mediated apoptosis.
|Salas E,Roy S,Marsh T,Rubin B,Debnath J||Oncogene (35:2913)||2016|
A new water soluble MAPK activator exerts antitumor activity in melanoma cells resistant to the BRAF inhibitor vemurafenib.
44-680G was used in western blot to test if NBDHEX and MC3181 have antitumor activity against melanoma cells resistant to vemurafenib
|Graziani G,Artuso S,De Luca A,Muzi A,Rotili D,Scimeca M,Atzori MG,Ceci C,Mai A,Leonetti C,Levati L,Bonanno E,Tentori L,Caccuri AM||Biochemical pharmacology (95:16)||2015|
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
44-680G was used in western blot to uncover a role for ATG12-ATG3 in late endosome function that is distinct from autophagy.
|Murrow L,Malhotra R,Debnath J||Nature cell biology (17:300)||2015|
Silver and fullerene nanoparticles' effect on interleukin-2-dependent proliferation of CD4 (+) T cells.
44-680G was used in western blot to determine if nanoparticles inhibit IL-2-dependent T cell proliferation.
|Côté-Maurais G,Bernier J||Toxicology in vitro : an international journal published in association with BIBRA (28:1474)||2014|
Increased ERK and JNK activation and decreased ERK/JNK ratio are associated with long-term organ damage in patients with systemic lupus erythematosus.
44-680G was used in western blot to test if extracellular signal-regulated kinase and c-Jun N-terminal kinase are associated with long-term organ damage in SLE patients.
|Bloch O,Amit-Vazina M,Yona E,Molad Y,Rapoport MJ||Rheumatology (Oxford, England) (53:1034)||2014|
The C-terminus of human nucleotide receptor P2X7 is critical for receptor oligomerization and N-linked glycosylation.
44-680G was used in western blot to alter the C-terminal trafficking domain of P2X7 and examine its function.
|Wickert LE,Blanchette JB,Waldschmidt NV,Bertics PJ,Denu JM,Denlinger LC,Lenertz LY||PloS one (8:null)||2013|
|Human||Not Cited||Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling.||Smeets RL,Fleuren WW,He X,Vink PM,Wijnands F,Gorecka M,Klop H,Bauerschmidt S,Garritsen A,Koenen HJ,Joosten I,Boots AM,Alkema W||BMC immunology (13:null)||2012|
|Human||Not Cited||Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.||Ratthé C,Pelletier M,Chiasson S,Girard D||Journal of leukocyte biology (81:1287)||2007|
|Human||Not Cited||The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.||Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R||Gynecologic oncology (102:453)||2006|
|Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization.||del Pozo MA,Balasubramanian N,Alderson NB,Kiosses WB,Grande-García A,Anderson RG,Schwartz MA||Nature cell biology (7:901)||2005|
|Human||Not Cited||CHIR-258: a potent inhibitor of FLT3 kinase in experimental tumor xenograft models of human acute myelogenous leukemia.||Lopes de Menezes DE,Peng J,Garrett EN,Louie SG,Lee SH,Wiesmann M,Tang Y,Shephard L,Goldbeck C,Oei Y,Ye H,Aukerman SL,Heise C||Clinical cancer research : an official journal of the American Association for Cancer Research (11:5281)||2005|
|Human||Not Cited||Mitogen-activated protein kinase expression and activation does not differentiate benign from malignant mesothelial cells.||Vintman L,Nielsen S,Berner A,Reich R,Davidson B||Cancer (103:2427)||2005|
A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis.
44-680G was used in immunocytochemistry to describe a method for the preparation, fixation, and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis
|Cheng Z,Liu F,Zhu S,Tian H,Wang L,Wang Y||PloS one (10:null)||2015|
|C. elegans||Not Cited||
TFG-1 function in protein secretion and oncogenesis.
44-680G was used in immunocytochemistry to identify and characterize the role of TFG-1 in protein secretion and export of cargo from the endoplasmic reticulum.
|Witte K,Schuh AL,Hegermann J,Sarkeshik A,Mayers JR,Schwarze K,Yates JR,Eimer S,Audhya A||Nature cell biology (13:550)||2011|
The MEK1/2-ERK1/2 pathway is activated in chronic rhinosinusitis with nasal polyps.
44-680G was used in immunohistochemistry and western blot to assess the role of the MEK/2-ERK/2 signaling pathway in the pathogenesis of chronic rhinosinusitis with nasal polyps.
|Linke R,Pries R,Könnecke M,Bruchhage KL,Böscke R,Gebhard M,Wollenberg B||Archivum immunologiae et therapiae experimentalis (62:217)||2014|
ERK-1; ERK-2; ERK1; ERK2; ERT1; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK3; microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 1a; mitogen-activated protein kinase 2; mitogen-activated protein kinase 3; p42-MAPK; p44 MAP kinase; p44-MAPK; pp42/MAP kinase; PRKM1; PRKM2; PRKM3; protein tyrosine kinase ERK2
9030612K14Rik; AA407128; AU018647; BOS_16809; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; mapk1a; MAPK2; MAPK3; MNK1; mpk1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3; xp42