Immunohistochemistry was performed on paraffin-embedded human colon carcinoma tissue. To expose target proteins, antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10 minutes. Following antigen retrieval, endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes, tissues were blocked in 5% normal goat serum in TBST for 1 hour at room temperature, and then probed with a Phospho-p44/42 MAPK (Erk1/2) (pThr202/Tyr204) monoclonal antibody (Product # MA5-15173) at a dilution of 1:400 overnight at 4°C. Detection was performed using an HRP-conjugated anti-rabbit IgG secondary reagent system followed by DAB substrate. Tissues were counterstained with Hematoxylin and visualized by light microscopy.
|Tested species reactivity||Pig, Rat, Yeast, Zebrafish, Fruit fly, Hamster, Human, Mink, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr202/Tyr204 of human p44 MAP kinase|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 - 3 µl|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.