Immunofluorescent analysis of Phospho-p44/42 MAPK/Erk1/2 pThr202/Tyr204 in NIH/3T3 cells stimulated with Platelet-Derived Growth Factor, using a Phospho-p44/42 MAPK/Erk1/2 pThr202/Tyr204 monoclonal antibody (Product # MA5-15174) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Fruit fly, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr202/Tyr204 of human p44 MAP kinase|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.