Western blot analysis of Phospho-p47phox pSer370 using Phospho-p47phox pSer370 polyclonal antibody (Product # PA5-36863) at a dilution of 1:500. Lane 1: Hela cells treated with serum starvation (24h), Lane 2: Raw264.7 cells treated with serum starvation (24h), Lane 3: H9C2 cells treated with serum starvation (24h).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human p47-phox around the phosphorylation site of Serine 370|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 45 kDa.
Purity is >95% by SDS-PAGE.
The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.