Western blots of 10 µg of PMA treated NIH 3T3 cell lysate (100 nM for fifteen minutes) showing specific immunolabeling of ~53 kDa p53 phosphorylated at Ser392. The labeling by the antibody was specifically blocked by the Ser392 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 of human TP53 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human and some non-human primates based on 100% sequence homology.
This antibody is specific for the ~53 kDa p53 protein phosphorylated at Ser392 in Western blots of NIH 3T3 cell lysates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
p53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002; Yin et al, 1992). It has been called the guardian of the genome (Lane, 1992). Phosphorylation of Ser392 in p53 is associated with formation of human tumors (Saito et al , 2003; Pise-Masison et al, 1998; Kim et al, 2004). In addition p53 has also been linked to affects of aging and oxidative stress (Sharpless and DePinho, 2002). An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al, 1998).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.