Western blot analysis of Phospho-p62 pSer28 in Jurkat lysate using a Phospho-p62 pSer28 polyclonal antibody (Product # PA5-35409). Results show a band at ~48Da. Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen but not by the corresponding dephosphopeptide (not shown).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser28 of human SQSTM1 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with non-human primate based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
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