Western blot analysis of Phospho-p70 S6 Kinase beta pSer423 in EGF-treated K562 whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-p70 S6 Kinase beta pSer423 polyclonal antibody (Product # PA5-35508).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthesized peptide derived from human p70 S6 Kinase beta around the phosphorylation site of Serine 423|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Phosphorylates specifically ribosomal protein S6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.