Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of Phospho-p70S6K Thr421/Ser424 in serum-starved HeLa cells treated with insulin (100ng/mL, 15 min) using a Phospho-p70S6K Thr421/Ser424 recombinant rabbit oligoclonal antibody (Product # 710108) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green), nuclei staining using DAPI (blue) and actin staining using Alexa Fluor 594 phalloidin (red). Images an And B are a composite image showing cytoplasmic and nuclear localization of phosphorylated p70S6K. Image C shows results of a competition assay using the phospho p70S6K (pTpS421/424) peptide.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide corresponding to amino acids 417–428 of human p70 S6K|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
p70 Ribosomal Protein S6 Kinase (p70S6K) is a 70 kDa member of the ribosomal S6 kinase (RSK) family of serine/threonine kinases. It is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70S6K acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p70(S6K)-alpha, S6K1, S6K, STK14A, p70-S6K, p70-alpha, PS6K, p70 S6KA, S6K-beta-1
p70 S6 kinase, alpha, ribosomal protein S6 kinase I, ribosomal protein S6 kinase beta-1, serine/threonine kinase 14 alpha, serine/threonine-protein kinase 14A, P70S6K1, S6K-beta-1, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, alpha 2, p70 S6K-alpha, p70 S6KA, p70-S6K 1