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Western blot analysis of phosphorylated p73 in (A) recombinant fusion protein containing phosphorylated tyrosine at position 99 and (B) fusion partner without this amino acid, using a Phospho-p73 pTyr99 polyclonal antibody (Product # PA1-41357) at 0.25 ug/ml.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues 75-125 of human p73, containing a phosphorylated tyrosine residue at position 99.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for TP73 (transient overexpression lysate).
p73 was identified as a long-lost cousin of tumor suppressor protein, p53 (4). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (3). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (2). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein (1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p53-like transcription factor; p53-related protein; P73