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|Tested species reactivity||Yeast|
|Published species reactivity||Yeast, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Yeast pGK (3-Phosphoglyceric Phosphokinase), EC 18.104.22.168|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5-10 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 21 publications below|
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of Telomere Length Requires a Conserved N-Terminal Domain of Rif2 in Saccharomyces cerevisiae.
459250 was used in western blot to study how telomere length is regulated.
|Kaizer H,Connelly CJ,Bettridge K,Viggiani C,Greider CW||Genetics (201:573)||2015|
Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNA segregation.
459250 was used in western blot to show that nuclear Rio1 integrates rDNA replication and segregation with ribosome biogenesis.
|Iacovella MG,Golfieri C,Massari LF,Busnelli S,Pagliuca C,Dal Maschio M,Infantino V,Visintin R,Mechtler K,Ferreira-Cerca S,De Wulf P||Nature communications (6:null)||2015|
Role of the unfolded protein response in regulating the mucin-dependent filamentous-growth mitogen-activated protein kinase pathway.
459250 was used in western blot to study the relationship between Msb2p and the unfolded protein response
|Adhikari H,Vadaie N,Chow J,Caccamise LM,Chavel CA,Li B,Bowitch A,Stefan CJ,Cullen PJ||Molecular and cellular biology (35:1414)||2015|
Growth-based determination and biochemical confirmation of genetic requirements for protein degradation in Saccharomyces cerevisiae.
459250 was used in western blot to describe a new yeast growth-based assay for identifying genetic requirements needed for protein degradation
|Watts SG,Crowder JJ,Coffey SZ,Rubenstein EM||Journal of visualized experiments : JoVE (null:null)||2015|
Evaluating the activity of the filamentous growth mitogen-activated protein kinase pathway in yeast.
459250 was used in western blot to describe three assays to measure the activity of the filamentous growth pathway.
|Cullen PJ||Cold Spring Harbor protocols (2015:276)||2015|
One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism.
459250 was used in western blot to describe a method to engineer the plasma membrane for research or therapy
|Swee LK,Lourido S,Bell GW,Ingram JR,Ploegh HL||ACS chemical biology (10:460)||2015|
Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway.
459250 was used in western blot to show that Bem4p is a new regulator of the filamentous-growth MAPK pathway
|Pitoniak A,Chavel CA,Chow J,Smith J,Camara D,Karunanithi S,Li B,Wolfe KH,Cullen PJ||Molecular and cellular biology (35:417)||2015|
Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.
459250 was used in western blot to show that the Rpb4/7 controls phosphorylation of the carboxy terminal domain of the Rpb1 subunit of RNAPII
|Allepuz-Fuster P,Martínez-Fernández V,Garrido-Godino AI,Alonso-Aguado S,Hanes SD,Navarro F,Calvo O||Nucleic acids research (42:13674)||2014|
Regulation of glucose-dependent gene expression by the RNA helicase Dbp2 in Saccharomyces cerevisiae.
459250 was used in western blot to investigate how Dbp2 integrates nutritional status and gene expression programs required for energy homeostasis
|Beck ZT,Cloutier SC,Schipma MJ,Petell CJ,Ma WK,Tran EJ||Genetics (198:1001)||2014|
Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.
459250 was used in western blot to show that Psh1 is phosphorylated by the Cka2 subunit of casein kinase 2 and promotes its E3 activity for Cse4
|Hewawasam GS,Mattingly M,Venkatesh S,Zhang Y,Florens L,Workman JL,Gerton JL||The Journal of biological chemistry (289:29297)||2014|
Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions.
459250 was used in western blot to assess if long-lived proteins exist in asymmetrically dividing cells and determine if they are involved in aging
|Thayer NH,Leverich CK,Fitzgibbon MP,Nelson ZW,Henderson KA,Gafken PR,Hsu JJ,Gottschling DE||Proceedings of the National Academy of Sciences of the United States of America (111:14019)||2014|
The nuclear exosome is active and important during budding yeast meiosis.
459250 was used in western blot to assess nuclear exosome activity during meiosis by deletion of TRF4
|Frenk S,Oxley D,Houseley J||PloS one (9:null)||2014|
The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.
459250 was used in western blot to investigate the role of Bud27 in RNA pol II transcription elongation
|Mirón-García MC,Garrido-Godino AI,Martínez-Fernández V,Fernández-Pevida A,Cuevas-Bermúdez A,Martín-Expósito M,Chávez S,de la Cruz J,Navarro F||Nucleic acids research (42:9666)||2014|
Essential genetic interactors of SIR2 required for spatial sequestration and asymmetrical inheritance of protein aggregates.
459250 was used in western blot to investigate the contribution of Sir2 to stress- and age-induced misfolded proteins deposition in aggregates and inclusion bodies
|Song J,Yang Q,Yang J,Larsson L,Hao X,Zhu X,Malmgren-Hill S,Cvijovic M,Fernandez-Rodriguez J,Grantham J,Gustafsson CM,Liu B,Nyström T||PLoS genetics (10:null)||2014|
Semi-quantitative colony immunoassay for determining and optimizing protein expression in Saccharomyces cerevisiae and Escherichia coli.
459250 was used in western blot to describe a fast semi-quantitative colony immunoassay to detect intracellularly expressed proteins in both yeast and bacterial cells.
|Cridge AG,Visweswaraiah J,Ramesh R,Sattlegger E||Analytical biochemistry (447:82)||2014|
Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis.
459250 was used in western blot to investigate the function of Aurora kinase in both the negative and positive regulation of spindle dynamics.
|Newnham L,Jordan PW,Carballo JA,Newcombe S,Hoffmann E||PloS one (8:null)||2014|
The lack of synchronization between iron uptake and cell growth leads to iron overload in Saccharomyces cerevisiae during post-exponential growth modes.
459250 was used in western blot to study iron import and growth rates in yeast.
|Park J,McCormick SP,Chakrabarti M,Lindahl PA||Biochemistry (52:9413)||2013|
Cytoplasmic localization of Hug1p, a negative regulator of the MEC1 pathway, coincides with the compartmentalization of Rnr2p-Rnr4p.
459250 was used in western blot to show the regulatory response of Hugp to DNA damage and suggest its role as a negative effector of the MEC pathway.
|Ainsworth WB,Hughes BT,Au WC,Sakelaris S,Kerscher O,Benton MG,Basrai MA||Biochemical and biophysical research communications (439:443)||2013|
Human copper chaperone for superoxide dismutase 1 mediates its own oxidation-dependent import into mitochondria.
459250 was used in western blot to elucidate how CCS is transported into mitochondria.
|Suzuki Y,Ali M,Fischer M,Riemer J||Nature communications (4:null)||2013|
tRNA tKUUU, tQUUG, and tEUUC wobble position modifications fine-tune protein translation by promoting ribosome A-site binding.
459250 was used in western blot to investigate the role of tRNA modifications in the modulation of wobbling capacity and translation efficiency.
|Rezgui VA,Tyagi K,Ranjan N,Konevega AL,Mittelstaet J,Rodnina MV,Peter M,Pedrioli PG||Proceedings of the National Academy of Sciences of the United States of America (110:12289)||2013|
Direct regulation of nucleosome density by the conserved AAA-ATPase Yta7.
459250 was used in western blot to elucidate how Yta7 contributes to breast cancer.
|Lombardi LM,Ellahi A,Rine J||Proceedings of the National Academy of Sciences of the United States of America (108:E1302)||2011|
cell migration-inducing gene 10 protein; MIG10; PGKA; primer recognition protein 2