Invitrogen

Phospholamban Monoclonal Antibody (2D12)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

40 Published Figures

153 References

View all (20) Phospholamban antibodies

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Phospholamban Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Phospholamban Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Phospholamban (Product # MA3-922) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Phospholamban Antibody in Western Blot (WB) — Western blot analysis of Phospholamban was performed by loading 25 µg of human heart (lane 1) and C2C12 (lane 2) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Phospholamban monoclonal antibody (Product # MA3-922) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~25 kDa.

Phospholamban Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 2 Repeated subcutaneous injection of PLN ASO in mice with PLN R14 Delta/Delta prevents cardiac dysfunction and improves survival. a Experimental design of the PLN R14 Delta/Delta intervention study, consisting of ASO#27 treatment with a 4-week loading phase with weekly dosing of 100 mg/kg and a 20-week maintenance phase with dosing of 50 mg/kg once every 4 weeks. Data points for functional and biochemical assessments were obtained at treatment week 4 (T4) which was the primary endpoint, 15 (T15), 19 (T19), and 23 (T23) at the end of the study. b SYBR green qPCR results of cardiac Pln mRNA expression ( n = 3 for wild-type [WT] and n = 4 for PBS and PLN-ASO). c Semi-quantified western blot analysis of PLN protein expression using LV protein lysates. Intensities were normalized to total protein loading control and the PBS treated mice ( n = 3 per group). d Immunofluorescence of WT, PBS treated and PLN-ASO treated PLN R14 Delta/Delta mice after 4 weeks treatment. Sections were co-stained for cardiac Troponin T (green), PLN (red), and DAPI (blue). Note the lower presence of visible PLN aggregations (white arrows) and the higher structural integrity of the PLN-ASO treated hearts vs. PBS control. Stainings were performed in two animals per group. e Representative MRI images after 4 weeks of treatment. f Quantification of LVEF (left ventricular ejection fraction), LVESV (left ventricular end-systolic volume), and LVEDV (left ventricular end-diastolic volume) ( n = 12 for PBS T4,

Phospholamban Antibody in Immunohistochemistry (IHC) — Figure 2 Histological and molecular analysis of PLN-R14del mice hearts. ( A ) Principal component analysis plot of RNA-Seq analysis of left ventricles of WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice of 3 or 8 weeks of age (n = 4 per group). 95% confidence ellipses of clusters are marked in grey. Information on genes contributing to variance in principle component 1 (PC1, x-axis) and principle component 2 (PC2, y-axis) is shown in Supplementary Fig. 1B . ( B ) qPCR measurements of left ventricular mRNA levels of Nppa (ANP), Nppb (BNP), and the ratio of Myh7 (beta-MHC) to Myh6 (alpha-MHC) of 8-week-old WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice (n = 4, 5, and 5, respectively). ( C ) Representative images of left ventricular sections stained with Masson's trichrome (scale bar = 70 mum) with ( D ) quantification of myocardial fibrosis in WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice hearts (n = 4, 5, and 5, respectively). ( E,F ) qPCR measurements of left ventricular mRNA levels of cardiac remodelling genes Col1a1 , Col1a2 , Col3a1 , Lgals3 (Gal-3), Timp1 , and Mmp2 of 8-week-old WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice (n = 4, 5, and 5, respectively). ( G ) Representative images of left ventricular sections of 8-week-old WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice stained with an anti-PLN antibody (red), wheat-germ agglutinin (WGA) (green), and DAPI (blue) (scale bar = 35 mum). ( H ) Western blot analysis of monomeric PLN proteins in RIPA-soluble and RI

Phospholamban Antibody — Fig 8 TRPC4beta attenuates hypertrophic growth in cardiomyocytes after GPCR stimulation. A, Hypertrophic growth of cardiomyocytes was measured as an increase of the cell surface after angiotensin II/phenylephrine (Ang II/PE) or vehicle (CON) treatment for 24 h. Red: alpha-actinin; Blue: DAPI. * P <0.05 vs Ad-betagal CON; $ P <0.05 vs Ad-C4beta AngII/PE. Cells from three different experiments were analyzed. Scale bar: 20 mum. N-number of cells is indicated in the bars. B, Expression and phosphorylation of phospholamban (PLN) and histone deacetylase 4 (HDAC4) in Ad-betagal and Ad-TRPC4beta NRCs after stimulation with Ang II/PE or vehicle (CON) for 24 h. phospho-PLN: PLN PT17; phospho-HDAC4: HDAC4 PS632; glyceraldehyde 3-phosphate dehydrogenase: GAPDH. Shown are representative immunoblots from three different experiments. C, Average quantified values of PLN PT17 signals expressed relative to Ad-betagal CON for n = 3 experiments. D, Average quantified values of HDAC4 PS632 signals expressed relative to Ad-betagal CON for n = 3 experiments. {TM}

Product Details

MA3-922

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	View 139 publications 139 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 5 publications 5 publications
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Chicken, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Rat
Published species	Dog, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Rodent
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	2D12
Immunogen	Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine Phospholamban. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2252716

Product Specific Information

MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse.

MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues.

The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.

Target Information

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue.

Stem cell reports

Shen S,Sewanan LR,Shao S,Halder SS,Stankey P,Li X,Campbell SG

MA3-922 was used in Western Blotting to present a method whereby maturation of hiPSC-CMs can be accelerated by simultaneous application of physiological Ca2+ and frequency-ramped electrical pacing in culture.

Tue Sep 13 00:00:00 EDT 2022

Interleukin-6-Mediated-Ca²⁺ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.

Frontiers in immunology

Liao J,Zhang S,Yang S,Lu Y,Lu K,Wu Y,Wu Q,Zhao N,Dong Q,Chen L,Du Y

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Feb 14 00:00:00 EST 2022

Interleukin-6-Mediated-Ca²⁺ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.

Frontiers in immunology

Liao J,Zhang S,Yang S,Lu Y,Lu K,Wu Y,Wu Q,Zhao N,Dong Q,Chen L,Du Y

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Feb 14 00:00:00 EST 2022

Phospholamban antisense oligonucleotides improve cardiac function in murine cardiomyopathy.

Nature communications

Grote Beverborg N,Später D,Knöll R,Hidalgo A,Yeh ST,Elbeck Z,Silljé HHW,Eijgenraam TR,Siga H,Zurek M,Palmér M,Pehrsson S,Albery T,Bomer N,Hoes MF,Boogerd CJ,Frisk M,van Rooij E,Damle S,Louch WE,Wang QD,Fritsche-Danielson R,Chien KR,Hansson KM,Mullick AE,de Boer RA,van der Meer P

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Aug 30 00:00:00 EDT 2021

Phospholamban antisense oligonucleotides improve cardiac function in murine cardiomyopathy.

Nature communications

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Aug 30 00:00:00 EDT 2021

Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel.

Nature communications

Liu D,Song AT,Qi X,van Vliet PP,Xiao J,Xiong F,Andelfinger G,Nattel S

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Immunoprecipitation

Wed May 05 00:00:00 EDT 2021

Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel.

Nature communications

Liu D,Song AT,Qi X,van Vliet PP,Xiao J,Xiong F,Andelfinger G,Nattel S

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Immunoprecipitation

Wed May 05 00:00:00 EDT 2021

Breast Cancer Promotes Cardiac Dysfunction Through Deregulation of Cardiomyocyte Ca²⁺-Handling Protein Expression That is Not Reversed by Exercise Training.

Journal of the American Heart Association

da Costa TSR,Urias U,Negrao MV,Jordão CP,Passos CS,Gomes-Santos IL,Salemi VMC,Camargo AA,Brum PC,Oliveira EM,Hajjar LA,Chammas R,Filho RK,Negrao CE

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Breast Cancer Promotes Cardiac Dysfunction Through Deregulation of Cardiomyocyte Ca²⁺-Handling Protein Expression That is Not Reversed by Exercise Training.

Journal of the American Heart Association

da Costa TSR,Urias U,Negrao MV,Jordão CP,Passos CS,Gomes-Santos IL,Salemi VMC,Camargo AA,Brum PC,Oliveira EM,Hajjar LA,Chammas R,Filho RK,Negrao CE

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Saxagliptin but Not Sitagliptin Inhibits CaMKII and PKC via DPP9 Inhibition in Cardiomyocytes.

Frontiers in physiology

Koyani CN,Trummer C,Shrestha N,Scheruebel S,Bourgeois B,Plastira I,Kickmaier S,Sourij H,Rainer PP,Madl T,Sattler W,Pelzmann B,Malle E,von Lewinski D

MA3-922 was used in Western Blotting to investigate whether dipeptidyl peptidase 8/9 contribute to saxagliptin-mediated inhibition of calmodulin-dependent protein kinase II and protein kinase C activity.

Sat Jan 09 00:00:00 EST 2021

Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.

Journal of molecular medicine (Berlin, Germany)

Pabel S,Reetz F,Dybkova N,Shomroni O,Salinas G,Mustroph J,Hammer KP,Hasenfuss G,Hamdani N,Maier LS,Streckfuss-Bömeke K,Sossalla S

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Dec 01 00:00:00 EST 2020

Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.

Journal of molecular medicine (Berlin, Germany)

Pabel S,Reetz F,Dybkova N,Shomroni O,Salinas G,Mustroph J,Hammer KP,Hasenfuss G,Hamdani N,Maier LS,Streckfuss-Bömeke K,Sossalla S

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Dec 01 00:00:00 EST 2020

Sex-specific cardiovascular responses to control or high fat diet feeding in C57bl/6 mice chronically exposed to bisphenol A.

Toxicology reports

Patel BB,Raad M,Sebag IA,Chalifour LE

MA3-922 was used in Western Blotting to show that chronic bisphenol A exposure alters cardiac contraction and relaxation and alters coordination of this contraction by the cardiac conduction system in mice.

Wed Sep 30 00:00:00 EDT 2020

Phosphorylation of cardiac myosin-binding protein-C contributes to calcium homeostasis.

The Journal of biological chemistry

Kumar M,Haghighi K,Kranias EG,Sadayappan S

MA3-922 was used in Western Blotting to conclude that improvement of contractile deficits alone without improving calcium handling may be insufficient for effective management of heart failure.

Fri Aug 07 00:00:00 EDT 2020

The phospholamban p.(Arg14del) pathogenic variant leads to cardiomyopathy with heart failure and is unreponsive to standard heart failure therapy.

Scientific reports

Eijgenraam TR,Boukens BJ,Boogerd CJ,Schouten EM,van de Kolk CWA,Stege NM,Te Rijdt WP,Hoorntje ET,van der Zwaag PA,van Rooij E,van Tintelen JP,van den Berg MP,van der Meer P,van der Velden J,Silljé HHW,de Boer RA

MA3-922 was used in Immunohistochemistry to generate a novel mouse model with the PLN-R14del pathogenic variant, performe detailed phenotyping, and test the efficacy of established heart failure therapies eplerenone or metoprolol.

Wed Jun 17 00:00:00 EDT 2020

The phospholamban p.(Arg14del) pathogenic variant leads to cardiomyopathy with heart failure and is unreponsive to standard heart failure therapy.

Scientific reports

Wed Jun 17 00:00:00 EDT 2020

Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase.

Circulation research

Xie D,Geng L,Xiong K,Zhao T,Wang S,Xue J,Wang C,Wang G,Feng Z,Zhou H,Li Y,Li L,Liu Y,Xue Z,Yang J,Ma H,Liang D,Chen YH

MA3-922 was used in Western Blot to indicate that cold-inducible RNA-binding protein acts as an mRNA stabilizer involved in the regulation of the heart rate stress response.

Fri Jun 05 00:00:00 EDT 2020

Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase.

Circulation research

Xie D,Geng L,Xiong K,Zhao T,Wang S,Xue J,Wang C,Wang G,Feng Z,Zhou H,Li Y,Li L,Liu Y,Xue Z,Yang J,Ma H,Liang D,Chen YH

MA3-922 was used in Western Blot to indicate that cold-inducible RNA-binding protein acts as an mRNA stabilizer involved in the regulation of the heart rate stress response.

Fri Jun 05 00:00:00 EDT 2020

Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts.

JCI insight

Liu T,Xiong F,Qi XY,Xiao J,Villeneuve L,Abu-Taha I,Dobrev D,Huang C,Nattel S

MA3-922 was used in Western Blotting to show reduced expression of total and phosphorylated ryanodine receptors and calsequestrin in AF and unchanged phospholamban/SERCA expression.

Tue Apr 07 00:00:00 EDT 2020

Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.

Stem cells (Dayton, Ohio)

Liang W,Han P,Kim EH,Mak J,Zhang R,Torrente AG,Goldhaber JI,Marbán E,Cho HC

MA3-922 was used in Western Blot to investigate the mechanisms for nodal pacemaker cell commitment.

Sun Mar 01 00:00:00 EST 2020

Nanoscale reorganization of sarcoplasmic reticulum in pressure-overload cardiac hypertrophy visualized by dSTORM.

Scientific reports

Hadipour-Lakmehsari S,Driouchi A,Lee SH,Kuzmanov U,Callaghan NI,Heximer SP,Simmons CA,Yip CM,Gramolini AO

MA3-922 was used in Western Blotting to investigate the changes in the nanoscale organisation of sarcoplasmic reticulum proteins in pathological cardiac hypertrophy.

Mon May 27 00:00:00 EDT 2019

Differential localizations of protein phosphatase 1 isoforms determine their physiological function in the heart.

Acta biochimica et biophysica Sinica

Liu R,Miller C,D'Annibale C,Vo K,Jacobs A

MA3-922 was used in Western Blotting to investigate the isoform-specific roles of protein phosphatase 1 in the heart.

Fri Mar 01 00:00:00 EST 2019

Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload.

Scientific reports

Miranda-Silva D,Gonçalves-Rodrigues P,Almeida-Coelho J,Hamdani N,Lima T,Conceição G,Sousa-Mendes C,González A,Díez J,Linke WA,Leite-Moreira A,Falcão-Pires I

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Wed Feb 27 00:00:00 EST 2019

Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload.

Scientific reports

Miranda-Silva D,Gonçalves-Rodrigues P,Almeida-Coelho J,Hamdani N,Lima T,Conceição G,Sousa-Mendes C,González A,Díez J,Linke WA,Leite-Moreira A,Falcão-Pires I

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Wed Feb 27 00:00:00 EST 2019

Regulation of BECN1-mediated autophagy by HSPB6: Insights from a human HSPB6^S10F mutant.

Autophagy

Liu GS,Zhu H,Cai WF,Wang X,Jiang M,Essandoh K,Vafiadaki E,Haghighi K,Lam CK,Gardner G,Adly G,Nicolaou P,Sanoudou D,Liang Q,Rubinstein J,Fan GC,Kranias EG

MA3-922 was used in Western Blotting to reveal a novel regulatory mechanism of heat shock protein family B [small] member 6 in cell survival through its interaction with BECN1.

Tue Feb 26 00:00:00 EST 2019

Effects of hypoestrogenism and/or hyperaldosteronism on myocardial remodeling in female mice.

Physiological reports

Rouet-Benzineb P,Merval R,Polidano E

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Thu Nov 01 00:00:00 EDT 2018

The DWORF micropeptide enhances contractility and prevents heart failure in a mouse model of dilated cardiomyopathy.

eLife

Makarewich CA,Munir AZ,Schiattarella GG,Bezprozvannaya S,Raguimova ON,Cho EE,Vidal AH,Robia SL,Bassel-Duby R,Olson EN

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Oct 09 00:00:00 EDT 2018

Empagliflozin Ammeliorates High Glucose Induced-Cardiac Dysfuntion in Human iPSC-Derived Cardiomyocytes.

Scientific reports

Ng KM,Lau YM,Dhandhania V,Cai ZJ,Lee YK,Lai WH,Tse HF,Siu CW

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Oct 05 00:00:00 EDT 2018

Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure.

Circulation

Kushnir A,Santulli G,Reiken SR,Coromilas E,Godfrey SJ,Brunjes DL,Colombo PC,Yuzefpolskaya M,Sokol SI,Kitsis RN,Marks AR

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Sep 11 00:00:00 EDT 2018

Three-dimensional imaging reveals endo(sarco)plasmic reticulum-containing invaginations within the nucleoplasm of muscle.

American journal of physiology. Cell physiology

Lee SH,Hadipour-Lakmehsari S,Miyake T,Gramolini AO

MA3-922 was used in Immunocytochemistry-immunoflourescence to characterise the molecular constituents of nuclear invaginations in the nucleoplasm in HEK293 cells, murine C2C12 muscle cells, and cardiac myocytes.

Thu Mar 01 00:00:00 EST 2018

Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity.

Arquivos brasileiros de cardiologia

Jacobsen BB,Leopoldo APL,Cordeiro JP,Campos DHS,Nascimento AFD,Sugizaki MM,Cicogna AC,Padovani CR,Leopoldo AS

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Wed Nov 01 00:00:00 EDT 2017

Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice.

PloS one

Fajardo VA,Gamu D,Mitchell A,Bloemberg D,Bombardier E,Chambers PJ,Bellissimo C,Quadrilatero J,Tupling AR

MA3922 was used in western blot to report the physiological role of sarcolipin upregulation in muscle myopathy

Wed Sep 06 00:00:00 EDT 2017

Increased passive stiffness promotes diastolic dysfunction despite improved Ca2+ handling during left ventricular concentric hypertrophy.

Cardiovascular research

Røe ÅT,Aronsen JM,Skårdal K,Hamdani N,Linke WA,Danielsen HE,Sejersted OM,Sjaastad I,Louch WE

MA3-922 was used in Western Blotting to dissect the contributions of passive and active mechanisms to diastolic dysfunction in the concentrically hypertrophied heart following pressure-overload.

Tue Aug 01 00:00:00 EDT 2017

Loss of type 9 adenylyl cyclase triggers reduced phosphorylation of Hsp20 and diastolic dysfunction.

Scientific reports

Li Y,Baldwin TA,Wang Y,Subramaniam J,Carbajal AG,Brand CS,Cunha SR,Dessauer CW

MA3-922 was used in Western Blotting to determine the physiological role of adenylyl cyclase type 9 in the heart.

Mon Jul 17 00:00:00 EDT 2017

TRPC4α and TRPC4β Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation.

PloS one

Kirschmer N,Bandleon S,von Ehrlich-Treuenstätt V,Hartmann S,Schaaf A,Lamprecht AK,Miranda-Laferte E,Langsenlehner T,Ritter O,Eder P

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Jul 10 00:00:00 EDT 2017

Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.

Human molecular genetics

Ravel-Chapuis A,Bélanger G,Côté J,Michel RN,Jasmin BJ

MA3-922 was used in Western Blotting to show that calcium-CnA signaling is hyperactivated in DM1 muscle and that such hyperactivation represents a beneficial compensatory adaptation to the disease.

Thu Jun 15 00:00:00 EDT 2017

High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β₂ -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts.

The Journal of physiology

Fu Q,Hu Y,Wang Q,Liu Y,Li N,Xu B,Kim S,Chiamvimonvat N,Xiang YK

MA3922 was used in western blot to evaluate the impacts of high fat diet on the insulin-adrenergic receptor signal network in hearts

Wed Mar 15 00:00:00 EDT 2017

Heart failure modulates electropharmacological characteristics of sinoatrial nodes.

Experimental and therapeutic medicine

Chang SL,Chuang HL,Chen YC,Kao YH,Lin YK,Yeh YH,Chen SA,Chen YJ

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Wed Feb 01 00:00:00 EST 2017

Genetically Encoded Biosensors Reveal PKA Hyperphosphorylation on the Myofilaments in Rabbit Heart Failure.

Circulation research

Barbagallo F,Xu B,Reddy GR,West T,Wang Q,Fu Q,Li M,Shi Q,Ginsburg KS,Ferrier W,Isidori AM,Naro F,Patel HH,Bossuyt J,Bers D,Xiang YK

MA3-922 was used in Western Blotting to show that PKA activity is shifted from the sarcolemma to the myofilaments in hypertrophic failing rabbit myocytes.

Fri Sep 30 00:00:00 EDT 2016

Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.

PloS one

Sadredini M,Danielsen TK,Aronsen JM,Manotheepan R,Hougen K,Sjaastad I,Stokke MK

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Sep 09 00:00:00 EDT 2016

Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.

Brain and behavior

Fajardo VA,Smith IC,Bombardier E,Chambers PJ,Quadrilatero J,Tupling AR

MA3-922 was used in western blot to study slow-twitch type 1 muscle fibers and diaphragm assessment in mice overexpressing phospholamban

Wed Jun 01 00:00:00 EDT 2016

Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats.

PloS one

Dalpiaz PL,Lamas AZ,Caliman IF,Ribeiro RF,Abreu GR,Moyses MR,Andrade TU,Gouvea SA,Alves MF,Carmona AK,Bissoli NS

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Apr 18 00:00:00 EDT 2016

Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins.

PloS one

Lugenbiel P,Wenz F,Govorov K,Schweizer PA,Katus HA,Thomas D

MA3-922 was used in Western Blotting to conclude that AF produced distinct arrhythmogenic remodeling of Ca2+ handling in the presence of tachycardia-induced mild HF that is different from AF without structural alterations.

Wed Jan 27 00:00:00 EST 2016

Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging.

American journal of physiology. Heart and circulatory physiology

Manning JR,Withers CN,Levitan B,Smith JD,Andres DA,Satin J

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Thu Oct 01 00:00:00 EDT 2015

Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.

Disease models & mechanisms

Fajardo VA,Bombardier E,McMillan E,Tran K,Wadsworth BJ,Gamu D,Hopf A,Vigna C,Smith IC,Bellissimo C,Michel RN,Tarnopolsky MA,Quadrilatero J,Tupling AR

MA3-922 was used in western blot to study SERCA dysfunction in mice overexpressing phospholamban

Sat Aug 01 00:00:00 EDT 2015

Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium.

Journal of molecular and cellular cardiology

Major JL,Salih M,Tuana BS

MA3-922 was used in western blot to study the relationship between the E2F pathway, beta2-adrenergic/PKA/PDE4D, and ERK/c-Src axis during the pathological hypertrophic growth response

Wed Jul 01 00:00:00 EDT 2015

Chronic exposure to low doses of HgCl2 avoids calcium handling impairment in the right ventricle after myocardial infarction in rats.

PloS one

Faria Tde O,Costa GP,Almenara CC,Angeli JK,Vassallo DV,Stefanon I,Vassallo PF

MA3-922 was used in western blot to study the normalization of right ventricular sarcolemmal calcium fluxes by chronic low-dose mercury chloride treatment following myocardial infarction in a rat model

Fri May 29 00:00:00 EDT 2015

Chronic lead exposure increases blood pressure and myocardial contractility in rats.

PloS one

Fioresi M,Simões MR,Furieri LB,Broseghini-Filho GB,Vescovi MV,Stefanon I,Vassallo DV

MA3-922 was used in western blot to study the elevated blood pressure and myocardial contractility of rats following chronic oral lead exposure

Wed Dec 31 00:00:00 EST 2014

Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes.

American journal of physiology. Cell physiology

Moshal KS,Zhang Z,Roder K,Kim TY,Cooper L,Patedakis Litvinov B,Lu Y,Reddy V,Terentyev D,Choi BR,Koren G

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Mon Dec 01 00:00:00 EST 2014

Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts.

PloS one

Sivagangabalan G,Nazzari H,Bignolais O,Maguy A,Naud P,Farid T,Massé S,Gaborit N,Varro A,Nair K,Backx P,Vigmond E,Nattel S,Demolombe S,Nanthakumar K

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Oct 17 00:00:00 EDT 2014

Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers.

PloS one

Fajardo VA,Bombardier E,Vigna C,Devji T,Bloemberg D,Gamu D,Gramolini AO,Quadrilatero J,Tupling AR

MA3-922 was used in immunohistochemistry - frozen section, immunoprecipitation, and western blot to determine if sarcolipin and phospholamban regulate SERCA1a, SERCA2a, or both in skeletal muscle

Tue Sep 16 00:00:00 EDT 2014

Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function.

Nature communications

Traister A,Li M,Aafaqi S,Lu M,Arab S,Radisic M,Gross G,Guido F,Sherret J,Verma S,Slorach C,Mertens L,Hui W,Roy A,Delgado-Olguín P,Hannigan G,Maynes JT,Coles JG

MA3-922 was used in Immunohistochemistry to show that integrin-linked kinase (ILK) mediates cardiomyocyte force transduction through regulation of sarcoplasmic/endoplasmic reticulum Ca(2+)ATPase isoform 2a (SERCA-2a) and phosphorylation of phospholamban (PLN) in the human heart.

Thu Sep 11 00:00:00 EDT 2014

The beneficial effects of ranolazine on cardiac function after myocardial infarction are greater in diabetic than in nondiabetic rats.

Journal of cardiovascular pharmacology and therapeutics

Mourouzis I,Mantzouratou P,Galanopoulos G,Kostakou E,Dhalla AK,Belardinelli L,Pantos C

MA3-922 was used in western blot to study the mechanisms underlying the greater therapeutic effect of ranolazine in diabetic than in non-diabetic infarcted rat heart

Mon Sep 01 00:00:00 EDT 2014

Aldosterone blockade reduces mortality without changing cardiac remodeling in spontaneously hypertensive rats.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Cezar MD,Damatto RL,Martinez PF,Lima AR,Campos DH,Rosa CM,Guizoni DM,Bonomo C,Cicogna AC,Gimenes R,Pagan LU,Okoshi MP,Okoshi K

MA3-922 was used in Western Blotting to evaluate the effects of the early administration of spironolactone on cardiac remodelling, myocardial function, and mortality in spontaneously hypertensive rats.

Thu Aug 14 00:00:00 EDT 2014

Measuring Ca2+-dependent Ca2+-uptake activity in the mouse heart.

Cold Spring Harbor protocols

Holemans T,Vandecaetsbeek I,Wuytack F,Vangheluwe P

MA3-922 was used in western blot to describe a protocol to determine the affinity of Ca(2+) to the Ca(2+) pump in crude homogenates of mouse heart.

Fri Aug 01 00:00:00 EDT 2014

Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation.

Arquivos brasileiros de cardiologia

Freire PP,Alves CA,Deus AF,Leopoldo AP,Leopoldo AS,Silva DC,Tomasi LC,Campos DH,Cicogna AC

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Jul 01 00:00:00 EDT 2014

Ryanodine receptor-mediated calcium leak drives progressive development of an atrial fibrillation substrate in a transgenic mouse model.

Circulation

Li N,Chiang DY,Wang S,Wang Q,Sun L,Voigt N,Respress JL,Ather S,Skapura DG,Jordan VK,Horrigan FT,Schmitz W,Müller FU,Valderrabano M,Nattel S,Dobrev D,Wehrens XHT

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Mar 25 00:00:00 EDT 2014

SERCA Cys674 sulphonylation and inhibition of L-type Ca2+ influx contribute to cardiac dysfunction in endotoxemic mice, independent of cGMP synthesis.

American journal of physiology. Heart and circulatory physiology

Hobai IA,Buys ES,Morse JC,Edgecomb J,Weiss EH,Armoundas AA,Hou X,Khandelwal AR,Siwik DA,Brouckaert P,Cohen RA,Colucci WS

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Oct 15 00:00:00 EDT 2013

Cardiac structure/function, protein expression, and DNA methylation are changed in adult female mice exposed to diethylstilbestrol in utero.

Canadian journal of physiology and pharmacology

Haddad R,Kasneci A,Sebag IA,Chalifour LE

MA3-922 was used in western blot to study the effects of in utero exposure to diethylstilbestrol on adult mouse cardiac biology

Sun Sep 01 00:00:00 EDT 2013

Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes.

American journal of physiology. Heart and circulatory physiology

Yi T,Vick JS,Vecchio MJ,Begin KJ,Bell SP,Delay RJ,Palmer BM

MA3-922 was used in western blot to study the mechanisms underlying the effects of zinc on cardiomyocyte contraction-relaxation

Sun Sep 01 00:00:00 EDT 2013

Carvedilol prevents ovariectomy-induced myocardial contractile dysfunction in female rat.

PloS one

Ribeiro RF,Potratz FF,Pavan BM,Forechi L,Lima FL,Fiorim J,Fernandes AA,Vassallo DV,Stefanon I

MA3-922 was used in western blot to study the mechanism underlying the ability of carvedilol to protect against dysfunctional cardiac contractile function in rats following ovariectomy

Mon Jul 01 00:00:00 EDT 2013

Inhibition of thyroid hormone receptor α1 impairs post-ischemic cardiac performance after myocardial infarction in mice.

Molecular and cellular biochemistry

Mourouzis I,Kostakou E,Galanopoulos G,Mantzouratou P,Pantos C

MA3-922 was used in western blot to study the deleterious effect of pharmacological inhibition of thyroid hormone receptor alpha1 on post-ischemic cardiac function in a murine model of myocardial infarction

Mon Jul 01 00:00:00 EDT 2013

Lifelong exposure to bisphenol a alters cardiac structure/function, protein expression, and DNA methylation in adult mice.

Toxicological sciences : an official journal of the Society of Toxicology

Patel BB,Raad M,Sebag IA,Chalifour LE

MA3-922 was used in western blot to study the effects on murine heart structure, function and molecular biology of lifelong exposure to polyphenol A

Wed May 01 00:00:00 EDT 2013

Right ventricular pacing with mechanical dyssynchrony causes apoptosis interruptus and calcium mishandling.

The Canadian journal of cardiology

Klug D,Boule S,Wissocque L,Montaigne D,Marechal X,Hassoun SM,Neviere R

MA3-922 was used in western blot to study the effects on left ventricular apoptosis and calcium metabolism of right ventricular pacing with mechanical dyssynchrony

Mon Apr 01 00:00:00 EDT 2013

Fosinopril attenuates the doxorubicin-induced cardiomyopathy by restoring the function of sarcoplasmic reticulum.

Cell biochemistry and biophysics

Zhang YC,Tang Y,Zhang M,Chen J,Zhou Q,Sun YG,Chen MT,Xu WP

MA3-922 was used in western blot to study the mechanism by which fosinapril protects against cardiomyopathy induced by treatment with doxorubicin

Sat Dec 01 00:00:00 EST 2012

Targeted sarcoplasmic reticulum Ca2+ ATPase 2a gene delivery to restore electrical stability in the failing heart.

Circulation

Cutler MJ,Wan X,Plummer BN,Liu H,Deschenes I,Laurita KR,Hajjar RJ,Rosenbaum DS

MA3-922 was used in western blot to study the restoration of electrical stability and contractile function of the failing heart by gene transfer of SERCA2a in a guinea pig model

Tue Oct 23 00:00:00 EDT 2012

Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?

Journal of cardiac failure

Middlekauff HR,Vigna C,Verity MA,Fonarow GC,Horwich TB,Hamilton MA,Shieh P,Tupling AR

MA3-922 was used in western blot to investigate the abnormalities of calcium handling proteins in skeletal and heart muscle during heart failure

Sat Sep 01 00:00:00 EDT 2012

Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction.

Circulation

Gurha P,Abreu-Goodger C,Wang T,Ramirez MO,Drumond AL,van Dongen S,Chen Y,Bartonicek N,Enright AJ,Lee B,Kelm RJ,Reddy AK,Taffet GE,Bradley A,Wehrens XH,Entman ML,Rodriguez A

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Jun 05 00:00:00 EDT 2012

Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.

American journal of physiology. Heart and circulatory physiology

Desroches BR,Zhang P,Choi BR,King ME,Maldonado AE,Li W,Rago A,Liu G,Nath N,Hartmann KM,Yang B,Koren G,Morgan JR,Mende U

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue May 15 00:00:00 EDT 2012

Cardiac response to doxorubicin and dexrazoxane in intact and ovariectomized young female rats at rest and after swim training.

American journal of physiology. Heart and circulatory physiology

Calvé A,Haddad R,Barama SN,Meilleur M,Sebag IA,Chalifour LE

MA3-922 was used in Western Blotting to characterise doxorubicin cardiac damage, and determine the ability of dexrazoxane to reduce this damage.

Tue May 15 00:00:00 EDT 2012

Estradiol promotes sudden cardiac death in transgenic long QT type 2 rabbits while progesterone is protective.

Heart rhythm

Odening KE,Choi BR,Liu GX,Hartmann K,Ziv O,Chaves L,Schofield L,Centracchio J,Zehender M,Peng X,Brunner M,Koren G

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue May 01 00:00:00 EDT 2012

Sildenafil prevents and reverses transverse-tubule remodeling and Ca(2+) handling dysfunction in right ventricle failure induced by pulmonary artery hypertension.

Hypertension (Dallas, Tex. : 1979)

Xie YP,Chen B,Sanders P,Guo A,Li Y,Zimmerman K,Wang LC,Weiss RM,Grumbach IM,Anderson ME,Song LS

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Wed Feb 01 00:00:00 EST 2012

Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle.

The Journal of physiology

Sjåland C,Lunde PK,Swift F,Munkvik M,Ericsson M,Lunde M,Boye S,Christensen G,Ellingsen Ø,Sejersted OM,Andersson KB

MA3-922 was used in western blot to investigate the role of SERCAs in muscle contractile function

Thu Dec 15 00:00:00 EST 2011

Baroreflex sensitivity impairment is associated with cardiac diastolic dysfunction in rats.

Journal of cardiac failure

Mostarda C,Moraes-Silva IC,Moreira ED,Medeiros A,Piratello AC,Consolim-Colombo FM,Caldini EG,Brum PC,Krieger EM,Irigoyen MC

MA3-922 was used in western blot to investigate the relationship between baroreflex dysfunction and heart diastolic function in rodents

Wed Jun 01 00:00:00 EDT 2011

Myocardial contractility is preserved early but reduced late after ovariectomy in young female rats.

Reproductive biology and endocrinology : RB&E

Paigel AS,Ribeiro RF,Fernandes AA,Targueta GP,Vassallo DV,Stefanon I

MA3-922 was used in western blot to investigate the effect of deficiency of ovarian sex hormones on myocardial contractility in rats after ovariectomy

Sat Apr 23 00:00:00 EDT 2011

cGMP signals modulate cAMP levels in a compartment-specific manner to regulate catecholamine-dependent signaling in cardiac myocytes.

Circulation research

Stangherlin A,Gesellchen F,Zoccarato A,Terrin A,Fields LA,Berrera M,Surdo NC,Craig MA,Smith G,Hamilton G,Zaccolo M

MA3-922 was used in Western Blotting to study the effect of cGMP signals on the local cAMP response to catecholamines in specific subcellular compartments.

Fri Apr 15 00:00:00 EDT 2011

Angiotensin receptor blockade improves the net balance of cardiac Ca(2+) handling-related proteins in sympathetic hyperactivity-induced heart failure.

Life sciences

Ferreira JC,Moreira JB,Campos JC,Pereira MG,Mattos KC,Coelho MA,Brum PC

MA3-922 was used in western blot to study the effects on Ca(2+) metabolism of therapeutic blockade of angiotensin II type 1 receptors.

Mon Mar 28 00:00:00 EDT 2011

Prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation.

Journal of cardiovascular electrophysiology

Alcalai R,Wakimoto H,Arad M,Planer D,Konno T,Wang L,Seidman JG,Seidman CE,Berul CI

MA3-922 was used in western blot to identify therapeutic targets in ventricular arrhythmia and calcium dysregulation

Tue Mar 01 00:00:00 EST 2011

Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes.

American journal of physiology. Heart and circulatory physiology

Horiuchi-Hirose M,Kashihara T,Nakada T,Kurebayashi N,Shimojo H,Shibazaki T,Sheng X,Yano S,Hirose M,Hongo M,Sakurai T,Moriizumi T,Ueda H,Yamada M

MA3-922 was used in western blot to investigate the mechanism for the decrease of the TT LTCC current density in failing ventricular myocytes

Tue Mar 01 00:00:00 EST 2011

cAMP- and Ca²(+) /calmodulin-dependent protein kinases mediate inotropic, lusitropic and arrhythmogenic effects of urocortin 2 in mouse ventricular myocytes.

British journal of pharmacology

Yang LZ,Kockskämper J,Khan S,Suarez J,Walther S,Doleschal B,Unterer G,Khafaga M,Mächler H,Heinzel FR,Dillmann WH,Pieske B,Spiess J

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Sat Jan 01 00:00:00 EST 2011

Silencing calcineurin A subunit reduces SERCA2 expression in cardiac myocytes.

American journal of physiology. Heart and circulatory physiology

Prasad AM,Inesi G

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Sat Jan 01 00:00:00 EST 2011

Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure.

Circulation

van Oort RJ,McCauley MD,Dixit SS,Pereira L,Yang Y,Respress JL,Wang Q,De Almeida AC,Skapura DG,Anderson ME,Bers DM,Wehrens XH

MA3-922 was used in western blot to investigate the involvement of ryanodine receptor phosphorylation in ventricular arrhythmias

Tue Dec 21 00:00:00 EST 2010

Cerebral ischemia elicits aberration in myocardium contractile function and intracellular calcium handling.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Sun L,Ai J,Wang N,Zhang R,Li J,Zhang T,Wu W,Hang P,Lu Y,Yang B

MA3-922 was used in western blot to investigate the effect of cerebral ischemia on myocardial dysfunction and calcium homeostasis

Wed Dec 08 00:00:00 EST 2010

Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction.

Molecular and cellular biochemistry

Kalofoutis C,Mourouzis I,Galanopoulos G,Dimopoulos A,Perimenis P,Spanou D,Cokkinos DV,Singh J,Pantos C

MA3-922 was used in western blot to investigate the effect of thyroid hormone on remodelling the diabetic myocardium

Wed Dec 01 00:00:00 EST 2010

More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease.

Journal of muscle research and cell motility

Hamdani N,Borbély A,Veenstra SP,Kooij V,Vrydag W,Zaremba R,Dos Remedios C,Niessen HW,Michel MC,Paulus WJ,Stienen GJ,van der Velden J

MA3-922 was used in western blot to investigate the different phenotypes between idiopathic dilated cardiomyopathy and ischemic heart disease

Wed Dec 01 00:00:00 EST 2010

Superior calcium homeostasis of extraocular muscles.

Experimental eye research

Zeiger U,Mitchell CH,Khurana TS

MA3-922 was used in western blot to investigate the effect of calcium buffering on extraocular muscles

Mon Nov 01 00:00:00 EDT 2010

Caspase-dependent protein phosphatase 2A activation contributes to endotoxin-induced cardiomyocyte contractile dysfunction.

Critical care medicine

Neviere R,Hassoun SM,Decoster B,Bouazza Y,Montaigne D,Maréchal X,Marciniak C,Marchetti P,Lancel S

MA3-922 was used in western blot to investigate the mechanism of intracellular calcium dysregulation caused by endotoxin

Fri Oct 01 00:00:00 EDT 2010

NADPH oxidase inhibition ameliorates cardiac dysfunction in rabbits with heart failure.

Molecular and cellular biochemistry

Liu Y,Huang H,Xia W,Tang Y,Li H,Huang C

MA3-922 was used in western blot to investigate the therapeutic effectiveness of NADPH oxidase inhibitor apocynin against heart failure in rabbits

Fri Oct 01 00:00:00 EDT 2010

Role of CaMKIIdelta phosphorylation of the cardiac ryanodine receptor in the force frequency relationship and heart failure.

Proceedings of the National Academy of Sciences of the United States of America

Kushnir A,Shan J,Betzenhauser MJ,Reiken S,Marks AR

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Tue Jun 01 00:00:00 EDT 2010

Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.

Neuromuscular disorders : NMD

Di Blasi C,Blasevich F,Bellafiore E,Mottarelli E,Gibertini S,Zanotti S,Saredi S,Mantegazza R,Morandi L,Mora M

MA3-922 was used in immunohistochemistry to investigate the immunohistochemical features of hexagonally cross-linked tubular arrays myopathy

Sat May 01 00:00:00 EDT 2010

Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway.

Cell calcium

Aguiar CJ,Andrade VL,Gomes ER,Alves MN,Ladeira MS,Pinheiro AC,Gomes DA,Almeida AP,Goes AM,Resende RR,Guatimosim S,Leite MF

MA3-922 was used in western blot to study the role of succinate in calcium homeostasis and cardiomyocyte viability

Fri Jan 01 00:00:00 EST 2010

Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration.

American journal of physiology. Heart and circulatory physiology

Wang J,Chan TO,Zhang XQ,Gao E,Song J,Koch WJ,Feldman AM,Cheung JY

MA3-922 was used in western blot to study the phenotype of transgenic rats overexpressing sodium/calcium exchanger .

Sat Aug 01 00:00:00 EDT 2009

Effects of thapsigargin and phenylephrine on calcineurin and protein kinase C signaling functions in cardiac myocytes.

American journal of physiology. Cell physiology

Prasad AM,Inesi G

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri May 01 00:00:00 EDT 2009

Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulation.

Journal of applied physiology (Bethesda, Md. : 1985)

Shao CH,Wehrens XH,Wyatt TA,Parbhu S,Rozanski GJ,Patel KP,Bidasee KR

MA3-922 was used in Western Blotting to show that ExT during diabetes normalizes RyR2 function and Ca(2+) release from the sarcoplasmic reticulum, providing insights into mechanisms by which ExT during diabetes improves cardiac function.

Wed Apr 01 00:00:00 EDT 2009

Electrophysiological consequences of dyssynchronous heart failure and its restoration by resynchronization therapy.

Circulation

Aiba T,Hesketh GG,Barth AS,Liu T,Daya S,Chakir K,Dimaano VL,Abraham TP,O'Rourke B,Akar FG,Kass DA,Tomaselli GF

MA3-922 was used in Western Blotting to study how CRT partially restores DHF-induced ion channel remodeling and abnormal Ca(2+) homeostasis and attenuates the regional heterogeneity of action potential duration.

Tue Mar 10 00:00:00 EDT 2009

Increased Ca2+ sensitivity of the ryanodine receptor mutant RyR2R4496C underlies catecholaminergic polymorphic ventricular tachycardia.

Circulation research

Fernández-Velasco M,Rueda A,Rizzi N,Benitah JP,Colombi B,Napolitano C,Priori SG,Richard S,Gómez AM

MA3-922 was used in Western Blotting to analyze the underlying Ca(2+) release defect that leads to arrhythmia in cardiomyocytes isolated from heterozygous knock-in mice carrying the RyR2(R4496C) mutation.

Fri Jan 30 00:00:00 EST 2009

Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases.

The Journal of biological chemistry

Prasad V,Bodi I,Meyer JW,Wang Y,Ashraf M,Engle SJ,Doetschman T,Sisco K,Nieman ML,Miller ML,Lorenz JN,Shull GE

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Nov 14 00:00:00 EST 2008

Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases.

The Journal of biological chemistry

Prasad V,Bodi I,Meyer JW,Wang Y,Ashraf M,Engle SJ,Doetschman T,Sisco K,Nieman ML,Miller ML,Lorenz JN,Shull GE

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Nov 14 00:00:00 EST 2008

Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases.

The Journal of biological chemistry

Prasad V,Bodi I,Meyer JW,Wang Y,Ashraf M,Engle SJ,Doetschman T,Sisco K,Nieman ML,Miller ML,Lorenz JN,Shull GE

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Nov 14 00:00:00 EST 2008

Nonionic detergent phase extraction for the proteomic analysis of heart membrane proteins using label-free LC-MS.

Proteomics

Donoghue PM,Hughes C,Vissers JP,Langridge JI,Dunn MJ

MA3-922 was used in western blot to evaluate a proteomic method for heart membrane proteins

Mon Sep 01 00:00:00 EDT 2008

Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model.

Circulation research

Rizzi N,Liu N,Napolitano C,Nori A,Turcato F,Colombi B,Bicciato S,Arcelli D,Spedito A,Scelsi M,Villani L,Esposito G,Boncompagni S,Protasi F,Volpe P,Priori SG

MA3-922 was used in western blot to investigate the structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin in a homozygous CASQ2 (R33Q/R33Q) knock in mouse model

Fri Aug 01 00:00:00 EDT 2008

Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* protein.

American journal of physiology. Heart and circulatory physiology

Zhu M,Gach AA,Liu G,Xu X,Lim CC,Zhang JX,Mao L,Chuprun K,Koch WJ,Liao R,Koren G,Blaxall BC,Mende U

MA3-922 was used in western blot to investigate the role of G alpha o on calcium cycling and contractile function in heart

Sat Mar 01 00:00:00 EST 2008

Myosin regulatory light chain E22K mutation results in decreased cardiac intracellular calcium and force transients.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Szczesna-Cordary D,Jones M,Moore JR,Watt J,Kerrick WG,Xu Y,Wang Y,Wagg C,Lopaschuk GD

MA3-922 was used in western blot to study how the myosin regulatory light chain E22K mutation affects its functions

Sat Dec 01 00:00:00 EST 2007

Serotonin increases L-type Ca2+ current and SR Ca2+ content through 5-HT4 receptors in failing rat ventricular cardiomyocytes.

American journal of physiology. Heart and circulatory physiology

Birkeland JA,Swift F,Tovsrud N,Enger U,Lunde PK,Qvigstad E,Levy FO,Sejersted OM,Sjaastad I

MA3-922 was used in western blot to study how 5-HT affects intracellular calcium handling

Mon Oct 01 00:00:00 EDT 2007

Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Pantos C,Mourouzis I,Markakis K,Dimopoulos A,Xinaris C,Kokkinos AD,Panagiotou M,Cokkinos DV

MA3-922 was used in western blot to investigate the role of thyroid hormone on cardiac remodeling and myocardial performance in rat

Wed Aug 01 00:00:00 EDT 2007

Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms.

American journal of physiology. Heart and circulatory physiology

Armoundas AA,Rose J,Aggarwal R,Stuyvers BD,O'rourke B,Kass DA,Marbán E,Shorofsky SR,Tomaselli GF,William Balke C

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Thu Mar 01 00:00:00 EST 2007

Identification of a novel phosphorylation site in protein phosphatase inhibitor-1 as a negative regulator of cardiac function.

The Journal of biological chemistry

Rodriguez P,Mitton B,Waggoner JR,Kranias EG

MA3-922 was used in western blot to study the mechanism for cardiac contractility regulation

Fri Dec 15 00:00:00 EST 2006

UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice.

The Journal of biological chemistry

Ukropec J,Anunciado RP,Ravussin Y,Hulver MW,Kozak LP

MA3-922 was used in western blot to investigate the thermogenic mechanism of the white adipose tissue in cold-acclimated Ucp1-/- mice.

Fri Oct 20 00:00:00 EDT 2006

Increased cardiac adenylyl cyclase expression is associated with increased survival after myocardial infarction.

Circulation

Takahashi T,Tang T,Lai NC,Roth DM,Rebolledo B,Saito M,Lew WY,Clopton P,Hammond HK

MA3-922 was used in western blot to investigate the relationship between left ventricular adenylyl cyclase type VI content and mortality after myocardial infarction

Tue Aug 01 00:00:00 EDT 2006

Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction.

Stem cells (Dayton, Ohio)

Dolnikov K,Shilkrut M,Zeevi-Levin N,Gerecht-Nir S,Amit M,Danon A,Itskovitz-Eldor J,Binah O

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Wed Feb 01 00:00:00 EST 2006

Protein kinase C and preconditioning: role of the sarcoplasmic reticulum.

American journal of physiology. Heart and circulatory physiology

Yamamura K,Steenbergen C,Murphy E

MA3-922 was used in western blot to study the mechanism for the cardioprotective effect of protein kinase C

Thu Dec 01 00:00:00 EST 2005

Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure.

Physiological genomics

Rothermel BA,Berenji K,Tannous P,Kutschke W,Dey A,Nolan B,Yoo KD,Demetroulis E,Gimbel M,Cabuay B,Karimi M,Hill JA

MA3-922 was used in western blot to investigate the activation of intracellular signaling pathways by graded degrees of hemodynamic stress

Wed Sep 21 00:00:00 EDT 2005

Impaired Ca2+ handling and contraction in cardiomyocytes from mice with a dominant negative thyroid hormone receptor alpha1.

Journal of molecular and cellular cardiology

Tavi P,Sjögren M,Lunde PK,Zhang SJ,Abbate F,Vennström B,Westerblad H

MA3-922 was used in western blot to study the role of thyroid hormone receptor alpha1 in calcium homeostasis and cardiomyocyte function

Fri Apr 01 00:00:00 EST 2005

Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies.

American journal of physiology. Heart and circulatory physiology

Coutu P,Metzger JM

MA3-922 was used in western blot to investigate the effects of parvalbumin (Parv) expression and sarco (endo) plasmic reticulum calcium-ATPase (SERCA2a) overexpression in the presence of beta-adrenergic stimulation

Tue Feb 01 00:00:00 EST 2005

Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction.

American journal of physiology. Heart and circulatory physiology

Nakayama M,Yan X,Price RL,Borg TK,Ito K,Sanbe A,Robbins J,Lorell BH

MA3-922 was used in western blot to investigate the effect of chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor on myocyte contractile function

Sat Jan 01 00:00:00 EST 2005

Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy.

Journal of the American College of Cardiology

Takaseya T,Ishimatsu M,Tayama E,Nishi A,Akasu T,Aoyagi S

MA3-922 was used in western blot to investigate the effects of mechanical unloading on cardiomyocytes from doxorubicin-induced cardiomyopathy

Tue Dec 07 00:00:00 EST 2004

Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction.

Circulation research

van der Velden J,Merkus D,Klarenbeek BR,James AT,Boontje NM,Dekkers DH,Stienen GJ,Lamers JM,Duncker DJ

MA3-922 was used in western blot to study the mechanism underlying the early impairment of LV beta-adrenergic responsiveness after myocardial infarction in vivo

Fri Nov 26 00:00:00 EST 2004

Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy.

American journal of physiology. Heart and circulatory physiology

Suarez J,Gloss B,Belke DD,Hu Y,Scott B,Dieterle T,Kim YK,Valencik ML,McDonald JA,Dillmann WH

MA3-922 was used in western blot to investigate the effects of overexpression of SERCA2a on cardiac function and calcium handling in mice

Mon Nov 01 00:00:00 EST 2004

The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm.

American journal of physiology. Cell physiology

Radzyukevich TL,Moseley AE,Shelly DA,Redden GA,Behbehani MM,Lingrel JB,Paul RJ,Heiny JA

MA3-922 was used in western blot to study the role of the sodium/potassium-ATPase alpha2-subunit isoform during muscle contraction

Mon Nov 01 00:00:00 EST 2004

Adenylyl cyclase type VI gene transfer reduces phospholamban expression in cardiac myocytes via activating transcription factor 3.

The Journal of biological chemistry

Gao MH,Tang T,Guo T,Sun SQ,Feramisco JR,Hammond HK

MA3-922 was used in western blot to demonstrate the effect of ACVI on has cAMP-independent gene transcription.

Fri Sep 10 00:00:00 EDT 2004

Phosphorylation-status of phospholamban and calsequestrin modifies their affinity towards commonly used antibodies.

Journal of molecular and cellular cardiology

Huke S,Periasamy M

MA3-922 was used in western blot to investigate the influence of phosphorylation on specific protein interaction with their cognate antibodies

Wed Sep 01 00:00:00 EDT 2004

Autologous stem cell transplantation for myocardial repair.

American journal of physiology. Heart and circulatory physiology

Liu J,Hu Q,Wang Z,Xu C,Wang X,Gong G,Mansoor A,Lee J,Hou M,Zeng L,Zhang JR,Jerosch-Herold M,Guo T,Bache RJ,Zhang J

MA3-922 was used in immunocytochemistry to evaluate the effect of a novel patch method for myocardial repair

Sun Aug 01 00:00:00 EDT 2004

Insights into cardioprotection obtained from study of cellular Ca2+ handling in myocardium of true hibernating mammals.

American journal of physiology. Heart and circulatory physiology

Yatani A,Kim SJ,Kudej RK,Wang Q,Depre C,Irie K,Kranias EG,Vatner SF,Vatner DE

MA3-922 was used in western blot to study mammalian hibernators' inborn cardioprotective functions during hibernation

Tue Jun 01 00:00:00 EDT 2004

Ca2+/calmodulin-dependent protein kinase II phosphorylation regulates the cardiac ryanodine receptor.

Circulation research

Wehrens XH,Lehnart SE,Reiken SR,Marks AR

MA3-922 was used in western blot to demonstrate that CaMKII phosphorylation could regulate the cardiac ryanodine receptor and increase RyR2 calcium sensitivity

Fri Apr 02 00:00:00 EST 2004

S100A1 gene transfer: a strategy to strengthen engineered cardiac grafts.

The journal of gene medicine

Remppis A,Pleger ST,Most P,Lindenkamp J,Ehlermann P,Schweda C,Löffler E,Weichenhan D,Zimmermann W,Eschenhagen T,Koch WJ,Katus HA

MA3-922 was used in western blot to evaluate the S100A1 gene transfer for engineering cardiac grafts

Thu Apr 01 00:00:00 EST 2004

Frequency- and afterload-dependent cardiac modulation in vivo by troponin I with constitutively active protein kinase A phosphorylation sites.

Circulation research

Takimoto E,Soergel DG,Janssen PM,Stull LB,Kass DA,Murphy AM

MA3-922 was used in western blot to elucidate the functional role of PKA-mediated phosphorylation of cTnI both in vivo and in vitro

Fri Mar 05 00:00:00 EST 2004

Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells.

Protein expression and purification

Waggoner JR,Huffman J,Griffith BN,Jones LR,Mahaney JE

MA3-922 was used in immunocytochemistry to investigate the use of the baculovirus/High-Five insect cell system for the recombinant expression of Ca(2+)-ATPase and phospholamban

Mon Mar 01 00:00:00 EST 2004

Accelerated onset of heart failure in mice during pressure overload with chronically decreased SERCA2 calcium pump activity.

American journal of physiology. Heart and circulatory physiology

Schultz Jel J,Glascock BJ,Witt SA,Nieman ML,Nattamai KJ,Liu LH,Lorenz JN,Shull GE,Kimball TR,Periasamy M

MA3-922 was used in western blot to investigate the effect of chronically decreased SERCA2 calcium pump activity on heart failure in mice

Mon Mar 01 00:00:00 EST 2004

Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart.

American journal of physiology. Heart and circulatory physiology

Zheng M,Dilly K,Dos Santos Cruz J,Li M,Gu Y,Ursitti JA,Chen J,Ross J,Chien KR,Lederer JW,Wang Y

MA3-922 was used in western blot to characterize the alterations in sarcoplasmic reticulum calcium homostasis in Ras-induced hypertrophic cardiomyopathy heart

Thu Jan 01 00:00:00 EST 2004

Gender differences in sarcoplasmic reticulum calcium loading after isoproterenol.

American journal of physiology. Heart and circulatory physiology

Chen J,Petranka J,Yamamura K,London RE,Steenbergen C,Murphy E

MA3-922 was used in western blot to study the possible relationship between SR calcium loading after isoproterenol and gender as well as its correlation with gender differences in ischemia-reperfusion injury

Mon Dec 01 00:00:00 EST 2003

Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?

Journal of molecular and cellular cardiology

Domenech RJ,Sánchez G,Donoso P,Parra V,Macho P

MA3-922 was used in western blot to study the effect of earlier tachycardia on infarct size

Mon Dec 01 00:00:00 EST 2003

Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt.

The Journal of biological chemistry

Kim YK,Kim SJ,Yatani A,Huang Y,Castelli G,Vatner DE,Liu J,Zhang Q,Diaz G,Zieba R,Thaisz J,Drusco A,Croce C,Sadoshima J,Condorelli G,Vatner SF

MA3-922 was used in western blot to investigate the effect of Akt on cardiac morphology and cardiac function.

Fri Nov 28 00:00:00 EST 2003

Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation.

The Journal of biological chemistry

Xu KY,Kuppusamy SP,Wang JQ,Li H,Cui H,Dawson TM,Huang PL,Burnett AL,Kuppusamy P,Becker LC

MA3-922 was used in western blot to demonstrate the protective effect of nitric oxide on cardiac SL NOSs and sodium/potassium-ATPase against ischemia-induced inactivation.

Fri Oct 24 00:00:00 EDT 2003

Regional alterations in protein expression in the dyssynchronous failing heart.

Circulation

Spragg DD,Leclercq C,Loghmani M,Faris OP,Tunin RS,DiSilvestre D,McVeigh ER,Tomaselli GF,Kass DA

MA3-922 was used in western blot to examine the regional changes in protein expression during heart failure

Tue Aug 26 00:00:00 EDT 2003

Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17.

The Journal of biological chemistry

Ji Y,Li B,Reed TD,Lorenz JN,Kaetzel MA,Dedman JR

MA3-922 was used in western blot to investigate the role of the calcium/calmodulin-dependent kinase II pathway in cardiac disease.

Fri Jul 04 00:00:00 EDT 2003

Interaction between increased SERCA2a activity and beta -adrenoceptor stimulation in adult rabbit myocytes.

American journal of physiology. Heart and circulatory physiology

Chaudhri B,Del Monte F,Hajjar RJ,Harding SE

MA3-922 was used in western blot to study the interaction of increased SERCA2a activity with beta-adrenoceptor stimulation in rabbit myocytes

Sun Dec 01 00:00:00 EST 2002

Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats.

American journal of physiology. Heart and circulatory physiology

Choi KM,Zhong Y,Hoit BD,Grupp IL,Hahn H,Dilly KW,Guatimosim S,Lederer WJ,Matlib MA

MA3-922 was used in western blot to study the possible contribution of defects in intracellular calcium signaling cardiomyopathy in streptozotocin (STZ)-induced diabetic rats

Tue Oct 01 00:00:00 EDT 2002

Altered dose response to beta-agonists in SERCA1a-expressing hearts ex vivo and in vivo.

American journal of physiology. Heart and circulatory physiology

Huke S,Prasad V,Nieman ML,Nattamai KJ,Grupp IL,Lorenz JN,Periasamy M

MA3-922 was used in western blot to investigate the contractility of hearts expressing SERCA1a ex vivo and in vivo as well as their response to beta-adrenergic stimulation

Sun Sep 01 00:00:00 EDT 2002

Developmental changes of Ca(2+) handling in mouse ventricular cells from early embryo to adulthood.

Life sciences

Liu W,Yasui K,Opthof T,Ishiki R,Lee JK,Kamiya K,Yokota M,Kodama I

MA3-922 was used in western blot to investigate the calcium handling in cardiomyocytes throughout murine development

Fri Aug 02 00:00:00 EDT 2002

Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hong CS,Cho MC,Kwak YG,Song CH,Lee YH,Lim JS,Kwon YK,Chae SW,Kim DH

MA3-922 was used in western blot to study junctin's functions in heart

Thu Aug 01 00:00:00 EDT 2002

Type 1 phosphatase, a negative regulator of cardiac function.

Molecular and cellular biology

Carr AN,Schmidt AG,Suzuki Y,del Monte F,Sato Y,Lanner C,Breeden K,Jing SL,Allen PB,Greengard P,Yatani A,Hoit BD,Grupp IL,Hajjar RJ,DePaoli-Roach AA,Kranias EG

MA3-922 was used in western blot to demonstrate the role of type 1 phosphatase in heart function.

Sat Jun 01 00:00:00 EDT 2002

Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy.

Diabetes

Trost SU,Belke DD,Bluhm WF,Meyer M,Swanson E,Dillmann WH

MA3-922 was used in western blot to investigate the effects of overexpression of the SR calcium pump (SERCA2a) on myocardial contractility in diabetic cardiomyopathy

Mon Apr 01 00:00:00 EST 2002

Targeting phospholamban by gene transfer in human heart failure.

Circulation

del Monte F,Harding SE,Dec GW,Gwathmey JK,Hajjar RJ

MA3-922 was used in immunocytochemistry to investigate that phospholamban may a good good therapeutic target in human heart failure.

Tue Feb 26 00:00:00 EST 2002

Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel.

Circulation research

Song LS,Guia A,Muth JN,Rubio M,Wang SQ,Xiao RP,Josephson IR,Lakatta EG,Schwartz A,Cheng H

MA3-922 was used in western blot to study the effects of increase in L-type calcium channels (LCC) density on calcium signaling in cardiac myocytes overexpressing the alpha (1) subunit of LCC

Fri Feb 08 00:00:00 EST 2002

Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart.

Circulation

Toma C,Pittenger MF,Cahill KS,Byrne BJ,Kessler PD

MA3-922 was used in immunohistochemistry to investigate the myogenic differentiation of human mesenchymal stem cells (hMSCs) in the adult murine heart

Tue Jan 01 00:00:00 EST 2002

mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.

Journal of cell science

Kapiloff MS,Jackson N,Airhart N

MA3-922 was used in western blot to study the role of mAKAP and ryanodine receptor in cardiomyocyte nuclear envelope signalling.

Sat Sep 01 00:00:00 EDT 2001

Altered SR protein expression associated with contractile dysfunction in diabetic rat hearts.

American journal of physiology. Heart and circulatory physiology

Zhong Y,Ahmed S,Grupp IL,Matlib MA

MA3-922 was used in western blot to investigate the relationship of altered sarcoplasmic reticulum (SR) protein expression with late-onset dysfunction in diabetic rats

Sat Sep 01 00:00:00 EDT 2001

Impaired sarcoplasmic reticulum function leads to contractile dysfunction and cardiac hypertrophy.

American journal of physiology. Heart and circulatory physiology

Meyer M,Trost SU,Bluhm WF,Knot HJ,Swanson E,Dillmann WH

MA3-922 was used in western blot to detect the expression changes of phospholamban in mices treated with ryanodine

Tue May 01 00:00:00 EDT 2001

Interactions between phospholamban and beta-adrenergic drive may lead to cardiomyopathy and early mortality.

Circulation

Dash R,Kadambi V,Schmidt AG,Tepe NM,Biniakiewicz D,Gerst MJ,Canning AM,Abraham WT,Hoit BD,Liggett SB,Lorenz JN,Dorn GW,Kranias EG

MA3-922 was used in western blot to investigate the role of phospholamban (PLB) in the development of cardiomyopathy and early mortality

Tue Feb 13 00:00:00 EST 2001

Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.

The Journal of physiology

Cavagna M,O'Donnell JM,Sumbilla C,Inesi G,Klein MG

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Sun Oct 01 00:00:00 EDT 2000

Sprint training normalizes Ca(2+) transients and SR function in postinfarction rat myocytes.

Journal of applied physiology (Bethesda, Md. : 1985)

Zhang LQ,Zhang XQ,Ng YC,Rothblum LI,Musch TI,Moore RL,Cheung JY

MA3-922 was used in western blot to investigate the effect of sprint training on calcium regulation and sarcoplasmic reticulum (SR) function in postinfarction rat myocytes

Sat Jul 01 00:00:00 EDT 2000

Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.

Biochimica et biophysica acta

Froemming GR,Murray BE,Harmon S,Pette D,Ohlendieck K

MA3-922 was used in western blot to study the expression of Ca(2+) regulatory membrane protein isoforms in skeletal, cardiac and neonatal muscle fibres

Thu Jun 01 00:00:00 EDT 2000

Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo.

The Journal of biological chemistry

Zhai J,Schmidt AG,Hoit BD,Kimura Y,MacLennan DH,Kranias EG

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Apr 07 00:00:00 EDT 2000

Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo.

The Journal of biological chemistry

Zhai J,Schmidt AG,Hoit BD,Kimura Y,MacLennan DH,Kranias EG

Published figure using Phospholamban monoclonal antibody (Product # MA3-922) in Western Blot

Fri Apr 07 00:00:00 EDT 2000

Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies.

Circulation research

O'Rourke B,Kass DA,Tomaselli GF,Kääb S,Tunin R,Marbán E

MA3-922 was used in western blot to demonstrate that the mechanisms of altered excitation-contraction coupling in canine are similar to those reported in human heart failure

Fri Mar 19 00:00:00 EST 1999

Nitric oxide synthase in cardiac sarcoplasmic reticulum.

Proceedings of the National Academy of Sciences of the United States of America

Xu KY,Huso DL,Dawson TM,Bredt DS,Becker LC

MA3-922 was used in immunohistochemistry to study the localization and function of the nitric oxide synthase in heart.

Tue Jan 19 00:00:00 EST 1999

In vivo phosphorylation of cardiac troponin I by protein kinase Cbeta2 decreases cardiomyocyte calcium responsiveness and contractility in transgenic mouse hearts.

The Journal of clinical investigation

Takeishi Y,Chu G,Kirkpatrick DM,Li Z,Wakasaki H,Kranias EG,King GL,Walsh RA

MA3-922 was used in western blot to investigate the mechanism underlying depressed cardiac function caused by PKC beta 2-mediated phosphorylation

Wed Jul 01 00:00:00 EDT 1998

Regulation of Ca2+ handling by phosphorylation status in mouse fast- and slow-twitch skeletal muscle fibers.

The American journal of physiology

Liu Y,Kranias EG,Schneider MF

MA3-922 was used in western blot to study the regulatory role of phosphorylation status on calcium handling in mouse fast- and slow-twitch skeletal muscle fibers

Mon Dec 01 00:00:00 EST 1997

Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching.

The Journal of biological chemistry

Hu P,Yin C,Zhang KM,Wright LD,Nixon TE,Wechsler AS,Spratt JA,Briggs FN

MA3-922 was used in western blot to investigate how coordinated expression of SERCA2a and phospholamban is achieved during skeletal muscle phenotype switching

Fri May 12 00:00:00 EDT 1995

Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.

Circulation

Movsesian MA,Karimi M,Green K,Jones LR

MA3-922 was used in western blot to investigate the expression of SERCA2 ATPase, calsequestrin and phospholamban in nonfailing and failing human left ventricular myocardium

Mon Aug 01 00:00:00 EDT 1994

The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.

The Journal of cell biology

Jorgensen AO,Shen AC,Arnold W,McPherson PS,Campbell KP

MA3-922 was used in immunohistochemistry to check the existence of ryanodine receptor in junctional SR and corbular SR

Mon Feb 01 00:00:00 EST 1993

Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase.

The Journal of biological chemistry

Briggs FN,Lee KF,Wechsler AW,Jones LR

MA3-922 was used in western blot to study the expression of the phospholamban protein in stimulated muscles and the corresponding functions

Fri Dec 25 00:00:00 EST 1992

Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes.

The American journal of physiology

Sham JS,Jones LR,Morad M

MA3-922 was used in blocking/activating experiment to investigate the effect of phospholamban on the regulation of calcium uptake in myocytes of mammals with beta-adrenergic stimulation

Tue Oct 01 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: cardiac CMH18; Cardiac phospholamban; PLB; unnamed protein product

Gene Aliases: CMD1P; CMH18; PLB; Plm; PLN

UniProt ID: (Chicken) P26677, (Rabbit) P61015, (Human) P26678, (Pig) P61013, (Dog) P61012, (Rat) P61016, (Mouse) P61014

Entrez Gene ID: (Chicken) 396378, (Rabbit) 100009299, (Sheep) 101102067, (Human) 5350, (Pig) 397421, (Dog) 414755, (Rat) 64672, (Mouse) 18821, (Guinea pig) 100721387

Function(s)

Process(es)

calcium channel regulator activity ATPase inhibitor activity enzyme inhibitor activity protein binding identical protein binding ATPase binding protein homodimerization activity

Phospholamban Monoclonal Antibody (2D12)

Product Details

MA3-922

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

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Bioinformatics

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