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      View (2) other Phospholamban antibodies

      Invitrogen

      Phospholamban Monoclonal Antibody (2D12)

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      Catalog #:MA3-922

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      Product Details

      TESTED APPLICATIONS DILUTION
      Immunocytochemistry (ICC) 1:10-1:100
      Immunofluorescence (IF) 1:10-1:100
      Immunohistochemistry (Paraffin) (IHC (P)) 1:200
      Immunoprecipitation (IP) Assay dependent
      Western Blot (WB) 1:500-1:5000
      PUBLISHED APPLICATIONS
      Western Blot (WB) See 92 publications below
      Immunohistochemistry (Frozen) (IHC (F)) See 1 publications below
      Immunohistochemistry (IHC) See 4 publications below
      Immunocytochemistry (ICC) See 3 publications below
      Blocking Assay (BLOCK) See 1 publications below
      Tested Species reactivity Dog, Chicken, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Rat
      Published species reactivity Dog , Rabbit , Rat , Pig , Rodent , Mouse , Human , Guinea pig
      Host / Isotype Mouse / IgG2a
      Class Monoclonal
      Type Antibody
      Clone 2D12
      Immunogen Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine Phospholamban.
      Conjugate Unconjugated
      Form Liquid
      Storage buffer ascites diluted in PBS
      Contains 0.05% sodium azide
      Storage conditions -20° C, Avoid Freeze/Thaw Cycles
      RRID AB_2252716

      Product Specific Information

      MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse.

      MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues.

      The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.

      Background/Target Information

      Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (101)

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      Bioinformatics

      Protein Aliases: Cardiac phospholamban; CMD1P; CMH18; phospholamban; PLB; PLN

      Gene Aliases: CMD1P; CMH18; PLB; Plm; PLN

      UniProt ID: (Human) P26678, (Dog) P61012, (Chicken) P26677, (Rabbit) P61015, (Pig) P61013, (Rat) P61016, (Mouse) Q3UQ75

      Entrez Gene ID: (Human) 5350, (Dog) 414755, (Chicken) 396378, (Rabbit) 100009299, (Pig) 397421, (Sheep) 101102067, (Rat) 64672, (Mouse) 18821

      Function(s) Blue Arrow Icon
      regulation of the force of heart contraction calcium ion transport Notch signaling pathway blood circulation regulation of heart contraction response to zinc ion negative regulation of heart rate regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion negative regulation of ATPase activity response to insulin response to testosterone negative regulation of catalytic activity cardiac muscle tissue development protein homooligomerization regulation of cytosolic calcium ion concentration regulation of calcium ion transport negative regulation of calcium ion transport relaxation of cardiac muscle regulation of ryanodine-sensitive calcium-release channel activity regulation of cardiac muscle cell contraction adrenergic receptor signaling pathway involved in heart process regulation of cardiac muscle cell membrane potential regulation of the force of heart contraction by cardiac conduction negative regulation of calcium ion import negative regulation of calcium ion transmembrane transporter activity negative regulation of calcium ion binding regulation of calcium-transporting ATPase activity negative regulation of calcium-transporting ATPase activity regulation of relaxation of cardiac muscle negative regulation of calcium ion import into sarcoplasmic reticulum regulation of cardiac conduction cellular calcium ion homeostasis regulation of relaxation of muscle regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum negative regulation of heart contraction regulation of calcium ion import
      Process(es) Blue Arrow Icon
      enzyme inhibitor activity
       calcium channel regulator activity
       protein binding
       ATPase inhibitor activity
       identical protein binding
       ATPase binding

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