Western blot of Phosphoserine in whole cell extract of rat brain cortex using a Phosphoserine monoclonal antibody (Product # MA1-90649). The antibody was developed with a Goat anti-mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.
|Tested species reactivity||Chemical|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment: (pS) conjugated to KLH.|
|Storage buffer||ascites diluted in 0.01M sodium phosphate, pH 7.6, with 0.25M NaCl, 15mg/ml BSA|
|Contains||0.01% thimerosal, 0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90649 detects Phosphoserine from various samples.
MA1-90649 has been successfully used in ELISA, Western blot applications.
The MA1-90649 immunogen is: Recombinant fragment: (pS) conjugated to KLH.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
For indirect ELISA use a dilution of 1:4000.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.