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|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Phosphoserine conjugated to KLH.|
|Storage buffer||PBS, pH 7.3, with PEG, sucrose|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Phosphorylated serine (phosphoserine) is an alpha amino acid that together with phosphothreonine, is one of the most ubiquitously modified amino acids in proteins. Many hormones, such as adrenaline (epinephrine), alter the activities of enzymes by stimulating the phosphorylation of serine and threonine, and these can have profound effects on cellular signal transduction, apoptosis and carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L.
44911M was used in western blot to demonstrate that G-protein-coupled receptors modulate the levels of Atg14L through ZBTB16.
|Zhang T,Dong K,Liang W,Xu D,Xia H,Geng J,Najafov A,Liu M,Li Y,Han X,Xiao J,Jin Z,Peng T,Gao Y,Cai Y,Qi C,Zhang Q,Sun A,Lipinski M,Zhu H,Xiong Y,Pandolfi PP,Li H,Yu Q,Yuan J||eLife (4:null)||2015|