ELISA of BSA conjugates of pT, pY and pS using a Phosphothreonine monoclonal antibody (Product # MA1-91728) followed by detection using an HRP-conjugated goat anti-mouse IgG (H and L) with TMB substrate. Each well was coated with 0.1µg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution.
|Tested species reactivity||Chemical|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Phosphothreonine conjugated KLH.|
|Storage buffer||ascites, pH 7.2|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:2000 - 1:10000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91728 detects Phosphothreonine from various samples.
MA1-91728 has been successfully used in Western blot and ELISA applications.
The MA1-91728 immunogen is: Phosphothreonine conjugated KLH.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.