|Tested species reactivity||Chemical|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||O-phospho-L-tyrosine and O-phospho-DL-tyramine|
|Storage buffer||PBS with 40% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 -1:200|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA4-003 detects phosphotyrosine from most species and tissues.
MA4-003 has been successfully used in ELISA, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and FACS procedures. This antibody detects proteins which contain phosphorylated tyrosine residues.
The MA4-003 antigen is O-phospho-L-tyrosine and O-phospho-DL-tyramine.
Protein kinases like platelet-derived growth factor and epidermal growth factor have the ability to phosphorylate tyrosine residues of other proteins like GTPase activating protein. Protein kinases play a prominent role in the regulation of normal growth as well as tumor growth. A number of retroviruses encode proteins with tyrosine kinase activity and infection with such a virus is associated with a 10-20 fold increase in phosphotyrosine on the virally encoded protein as well as on other cellular proteins. This antibody, from clone 1G2, is capable of specifically binding to proteins which contain phosphorylated tyrosine residues, but will not react with any other phosphorylated amino acids, proteins or nucleotides, and is excellent for the study of growth factor receptors, oncogenes, and signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
JNK and tumor necrosis factor-alpha mediate free fatty acid-induced insulin resistance in 3T3-L1 adipocytes.
MA4-003 was used in western blot to investigate the role of JNK and tumor necrosis factor-alpha in free fatty acid-induced insulin resistance in 3T3-L1 adipocytes.
|Nguyen MT,Satoh H,Favelyukis S,Babendure JL,Imamura T,Sbodio JI,Zalevsky J,Dahiyat BI,Chi NW,Olefsky JM||The Journal of biological chemistry (280:35361)||2005|
Cell lines and peripheral blood leukocytes derived from individuals with chronic myelogenous leukemia display virtually identical proteins phosphorylated on tyrosine residues.
MA4-003 was used in immunoprecipitation to investigate the important role of ABL protein possessing constitutive protein-tyrosine kinase activity in chronic myelogenous leukemia
|Huhn RD,Posner MR,Rayter SI,Foulkes JG,Frackelton AR||Proceedings of the National Academy of Sciences of the United States of America (84:4408)||1987|