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|Tested species reactivity||Chem|
|Published species reactivity||Rat, Primate, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Phosphotyrosine coupled to carrier protein.|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is A431 cells stimulated with EGF.
Protein kinases like platelet-derived growth factor and epidermal growth factor have the ability to phosphorylate tyrosine residues of other proteins like GTPase activating protein. Protein kinases play a prominent role in the regulation of normal growth as well as tumor growth. A number of retroviruses encode proteins with tyrosine kinase activity and infection with such a virus is associated with a 10-20 fold increase in phosphotyrosine on the virally encoded protein as well as on other cellular proteins. This antibody, from clone 1G2, is capable of specifically binding to proteins which contain phosphorylated tyrosine residues, but will not react with any other phosphorylated amino acids, proteins or nuecleotides, and is excellent for the study of growth factor receptors, oncogenes, and signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Imatinib as a key inhibitor of the platelet-derived growth factor receptor mediated expression of cell surface heparan sulfate proteoglycans and functional properties of breast cancer cells.
MA1-12445 was used in western blot to study the effects of Glivec on breast cancer heparin sulfate proteogycan expression and cell proliferation
|Malavaki CJ,Roussidis AE,Gialeli C,Kletsas D,Tsegenidis T,Theocharis AD,Tzanakakis GN,Karamanos NK||The FEBS journal (280:2477)||2013|
RhoH plays critical roles in Fc epsilon RI-dependent signal transduction in mast cells.
MA1-12445 was used in western blot to study the role of RhoH in Fc epsilon RI-dependent signal transduction in mast cells
|Oda H,Fujimoto M,Patrick MS,Chida D,Sato Y,Azuma Y,Aoki H,Abe T,Suzuki H,Shirai M||Journal of immunology (Baltimore, Md. : 1950) (182:957)||2009|
The combi-targeting concept: dissection of the binary mechanism of action of the combi-triazene SMA41 in vitro and antitumor activity in vivo.
MA1-12445 was used in western blot to study the anti-tumor mechanism of a unimolecular combination of an EGFR tyrosine kinase inhibitor and a DNA damaging triazine
|Matheson SL,McNamee JP,Wang T,Alaoui-Jamali MA,Tari AM,Jean-Claude BJ||The Journal of pharmacology and experimental therapeutics (311:1163)||2004|
A fully human monoclonal antibody to the insulin-like growth factor I receptor blocks ligand-dependent signaling and inhibits human tumor growth in vivo.
MA1-12445 was used in western blot to investigate the effectiveness of a humanized monoclonal insulin-like growth factor I receptor antibody against human tumor growth
|Burtrum D,Zhu Z,Lu D,Anderson DM,Prewett M,Pereira DS,Bassi R,Abdullah R,Hooper AT,Koo H,Jimenez X,Johnson D,Apblett R,Kussie P,Bohlen P,Witte L,Hicklin DJ,Ludwig DL||Cancer research (63:8912)||2003|
Inhibition of epidermal growth factor receptor-mediated signaling by "Combi-triazene" BJ2000, a new probe for Combi-Targeting postulates.
MA1-12445 was used in western blot to study the inhibition of EGFR-mediated signaling by a "Combi-triazene" molecule with binary EGFR targeting/DNA-damaging properties
|Brahimi F,Matheson SL,Dudouit F,McNamee JP,Tari AM,Jean-Claude BJ||The Journal of pharmacology and experimental therapeutics (303:238)||2002|
Functional implications of altered subcellular localization of PELP1 in breast cancer cells.
MA1-12445 was used in immunoprecipitation to investigate the role of altered subcellular localization of PELP1 in breast cancer cells
|Vadlamudi RK,Manavathi B,Balasenthil S,Nair SS,Yang Z,Sahin AA,Kumar R||Cancer research (65:7724)||2005|
Intracellular distribution of lysosomal sialidase is controlled by the internalization signal in its cytoplasmic tail.
MA1-12445 was used in immunoprecipitation to study the role of tyrosine phosphorylation of the cytoplasmic internalization signal of lysosomal sialidase in regulating its subcellular localization
|Lukong KE,Seyrantepe V,Landry K,Trudel S,Ahmad A,Gahl WA,Lefrancois S,Morales CR,Pshezhetsky AV||The Journal of biological chemistry (276:46172)||2001|
Delivery of insulin-like growth factor-I to the rat brain and spinal cord along olfactory and trigeminal pathways following intranasal administration.
MA1-12445 was used in immunohistochemistry to evaluate a specific method for IGF-I delivery into CNS system through intranasal administration
|Thorne RG,Pronk GJ,Padmanabhan V,Frey WH||Neuroscience (127:481)||2004|
Dual action of estrogen on glutamate-induced calcium signaling: mechanisms requiring interaction between estrogen receptors and src/mitogen activated protein kinase pathway.
MA1-12445 was used in immunocytochemistry to investigate the mechanism for the effect of estrogen on glutamate-induced calcium signaling
|Nilsen J,Chen S,Brinton RD||Brain research (930:216)||2002|