|Tested species reactivity||Chemical|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Phosphotyrosine conjugated to protein carriers.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. Milk-derived blocking solutions reportedly contain phosphoproteins that may inhibit phosphoamino acid antibody binding and therefore should be avoided. A suggested positive control is MCF-7 cells, EGF-stimulated A431 cells or breast carcinoma.
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. This antibody shows no cross-reaction with other phosphoamino acids and is excellent for multiple applications including staining of formalin/paraffin tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.