Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Chem|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine|
|Storage buffer||TBS with 40% glycerol, 1mM MgCl2, 1% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 2 publications below|
This antibody is known to show reactivity towards phosphothreonine.
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Phosphorylation of Tyr245 in the open-inhibited state of Abelson kinase does not induce downstream signaling.
03-7722 was used in ELISA to investigate the role of Tyr245 phosphorylation in Abelson kinase function
|Skora L,Kempf D,Mestan J,D'Orazio D,Jahnke W||European journal of haematology (96:502)||2016|
|Human||0.2 µg/ml||In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.||García-Echeverría C,Pearson MA,Marti A,Meyer T,Mestan J,Zimmermann J,Gao J,Brueggen J,Capraro HG,Cozens R,Evans DB,Fabbro D,Furet P,Porta DG,Liebetanz J,Martiny-Baron G,Ruetz S,Hofmann F||Cancer cell (5:231)||2004|