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Western blot detection of Phycoerythrin B in 1µg of (lane 1) B-Phycoerythrin reduced, (lane 2) B-Phycoerythrin reduced, (lane 3) control with no primary antibody using a Phycoerythrin B polyclonal antibody (Product # PA1-28741) at a dilution of 1:1000 following incubation overnight at 4°C and detected using a Dylight 649 conjugated Donkey anti-rabbit secondary antibody at a dilution of 1:10,000 for 1.5 hrs at room temperature.
|Tested species reactivity||Tag|
|Host / Isotype||Rabbit / IgG|
|Immunogen||B-Phycoerythrin [Porphyridium cruentum]|
|Storage buffer||whole serum diluted in 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28741 detects Phycoerythrin B from tagged samples.
PA1-28741 has been successfully used in ELISA, Western blot applications.
The PA1-28741 immunogen is: B-Phycoerythrin [Porphyridium cruentum]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.