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Pirh2 Antibody (MA5-37910) in WB
Western blot analysis of Pirh2 in extracts of HCT116 cells. Samples were incubated with Pirh2 Monoclonal antibody (Product # MA5-37910) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-37910
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4Q5H9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 160-261 of human RCHY1 (Q96PM5)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897830
Product Specific Information
Positive Samples: HCT116
Immunogen sequence: HVLPCGHLLH RTCYEEMLKE GYRCPLCMHS ALDMTRYWRQ LDDEVAQTPM PSEYQNMTVD ILCNDCNGRS TVQFHILGMK CKICESYNTA QAGGRRISLD QQ
Target Information
The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Androgen receptor N-terminal-interacting protein; androgen-receptor N-terminal-interacting protein; CH-rich interacting match with PLAG1; CH-rich-interacting match with PLAG1; DKFZp586C1620; E3 ubiquitin-protein ligase Pirh2; h PRO1996; hPirh2; p53-induced protein with a RING-H2 domain; p53-induced RING-H2 protein; PIRH2E; PIRH2F; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; Zinc finger protein 363; zinc finger, CHY-type
Gene Aliases: ARNIP; CHIMP; PIRH2; PRO1996; RCHY1; RNF199; ZCHY; Zfp363; ZNF363
UniProt ID: (Human) Q96PM5, (Mouse) Q9CR50
Entrez Gene ID: (Human) 25898, (Mouse) 68098
p53 binding
ubiquitin-protein transferase activity
receptor binding
protein binding
zinc ion binding
ligase activity
protein homodimerization activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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