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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified human placental alkaline phosphatase.|
|Purification||Ammonium sulfate precipitation, Ion-exchange chromatography|
|Storage buffer||PBS with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1/10- 1/40|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for immunohistochemical applications is placenta or seminoma. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
alkaline phosphatase Regan isozyme; alkaline phosphatase, placental type; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase 1; Regan isozyme
ALP; PALP; PLAP; PLAP-1