|Tested species reactivity||Dog, Human, Mouse|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to residues F(570) T P Q S R R L R E L P L A A D A L T F(589) of human plakophilin-3.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06511 detects plakophilin-3 in human, mouse, canine cells.
MA1-06511 has sucessfully been used in Western blotting, immunoprecipitation, immunocytochemistry and immunohistochemistry procedures. By Western blot it detects a ~87 kDa band representing Plakophilin-3.
The MA1-06511 immunogen is a synthetic peptide corresponding to residues F(570) T P Q S R R L R E L P L A A D A L T F(589) of human plakophilin-3.
Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate
staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Unimpaired skin carcinogenesis in Desmoglein 3 knockout mice.
MA1-06511 was used in immunocytochemistry to explore the role of desmosomal cadherins in carcinogenesis.
|Baron S,Hoang A,Vogel H,Attardi LD||PloS one (7:null)||2012|
E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly.
MA1-06511 was used in immunocytochemistry and western blot to test if plakophilin3 is required for desmosome formation
|Gosavi P,Kundu ST,Khapare N,Sehgal L,Karkhanis MS,Dalal SN||Cellular and molecular life sciences : CMLS (68:1439)||2011|
Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis.
MA1-06511 was used in western blot to investigate the effects of K8 filament stabilization
|Khapare N,Kundu ST,Sehgal L,Sawant M,Priya R,Gosavi P,Gupta N,Alam H,Karkhanis M,Naik N,Vaidya MM,Dalal SN||PloS one (7:null)||2012|
|Not Applicable||Not Cited||
The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.
MA1-06511 was used in ChIP assay, immunocytochemistry, immunohistochemistry, and western blot to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells
|Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A||FEBS letters (581:1617)||2007|