|Immunohistochemistry (Paraffin) (IHC (P))||4-6 ug/ml|
|Western Blot (WB)||0.1 - 0.3ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1 (NP_000593.1).|
|Storage buffer||TBS with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
Predicted to react with chimpanzee and dog based on sequence homology.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is human placenta. In Western blot, this antibody detects a band at ~45kDa in human duodenum, liver and lung lysates. A minimum incubation time of 1 hour is recommended with this antibody.
PAI-1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Endothelial plasminogen activator inhibitor; PAI; PAI-1; Plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Gene Aliases: PAI; PAI-1; PAI1; PLANH1; SERPINE1
UniProt ID: (Human) P05121
Entrez Gene ID: (Human) 5054
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