|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1 (NP_000593.1).|
|Storage buffer||TBS with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||4-6 ug/ml|
|Western Blot (WB)||0.1 - 0.3ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with chimpanzee and dog based on sequence homology.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is human placenta. In Western blot, this antibody detects a band at ~45kDa in human duodenum, liver and lung lysates. A minimum incubation time of 1 hour is recommended with this antibody.
PAI-1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.