|Tested species reactivity||Human|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human plasminogen protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
MA5-13846 targets Plasminogen in WB applications and shows reactivity with Human samples.
The MA5-13846 immunogen is human plasminogen protein.
Plasminogen is a key proenzyme of plasmin in the fibrinolytic and thrombolytic systems, the deficiency of which leads to a mild thrombotic tendency. Plasminogen binds to fibrin as well as to the surface of endothelial cells and monocytes/macrophages, where it is activated to plasmin. Streptokinase is a plasminogen activator widely used in treating blood-clotting disorders. Complexes of streptokinase with human plasminogen can hydrolytically activate other plasminogen molecules to plasmin, which then dissolves blood clots. Apolipoprotein(a) is very similar to plasminogen in terms of amino acid sequence, it inhibits plasmin generation by competing with plasminogen's binding to fibrin and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HGF regulates the activation of TGF-ß1 in rat hepatocytes and hepatic stellate cells.
MA5-13846 was used in ELISA to study the mechanism of HGF-regulated TGF-beta1 activation in hepatocytes and stellate cells and the significance for hepatic fibrosis
|Narmada BC,Chia SM,Tucker-Kellogg L,Yu H||Journal of cellular physiology (228:393)||2013|