|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues C D(127) L T S L R G (S/T) R P Y N Q R S(141) of human and rat Plasmolipin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-086 detects plasmolipin from rat and human samples.
PA1-086 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~18 kDa protein representing plasmolipin from rat kidney tissue extract.
The PA1-086 immunogen is a synthetic peptides corresponding to residues C D(127) L T S L R G (S/T) R P Y N Q R S(141) of human and rat Plasmolipin. This peptide (Cat. # PEP-250) can be used for neutralization and control experiments.
Plasmolipin is an 18 kDa membrane-bound proteolipid that belongs to the tetraspan (4TM) family, a rapidly growing group of myelin-associated proteins. Many of these proteins could be linked to human hereditary demyelinating neuropathies. Studies indicate that plasmolipin is a component of the cytoskeletal structure and has been localized to the apical surface of tubular epithelial cells. It has been shown that, upon addition to lipid bilayers, plasmolipin induces formation of ion channels that are both voltage-dependent and potassium-specific. Plasmolipin was originally isolated from kidney tissue. Subsequently, it has been shown to be widely expressed, not only being present in kidney, but also in the nervous system, thymus, testis, lung, and thyroid gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.